Expression of G protein-coupled receptor 30 in the spinal somatosensory system.
Expression of G protein-coupled receptor 30 in the spinal somatosensory system.
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DOI：
10.1016/j.brainres.2009.11.004
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发表时间：
2010-01-15
期刊：
影响因子：
2.9
通讯作者：
Kawata M
中科院分区：
文献类型：
--
作者：
Takanami K;Sakamoto H;Matsuda K;Hosokawa K;Nishi M;Prossnitz ER;Kawata M
雌激素最初被确定为女性中的主要性类固醇激素和生殖功能和性行为的调节剂，但长期以来一直认为雌激素对脊髓水平的体感系统也有局部影响。众所周知，雌激素的作用是由核雌激素受体（ER）通过基因组作用介导的，但最近，膜结合G蛋白偶联受体GPR30被鉴定为非基因组雌激素受体。本研究以ERα为对照，观察了大鼠脊髓和背根神经节（DRG）中GPR30的表达和定位。应用免疫组织化学和原位杂交技术，我们发现GPR30在雄性和雌性大鼠DRG神经元的表达在mRNA和蛋白水平上无性别差异。在脊髓背角外层观察到密集的GPR30免疫反应性积聚，并且选择性脊髓背根切断术揭示GPR30从DRG运输到位于脊髓背角的终末。GPR30在去卵巢大鼠背根节神经元表达下调。雌激素可能通过膜ER、GPR30介导的机制调节脊髓体感系统。
Estrogens were originally identified as the primary sex steroid hormones in females and regulators of reproductive function and sexual behavior, but it has long been suggested that estrogens also have local effects on the somatosensory system at the spinal cord level. It is well known that the effects of estrogens are mediated by nuclear estrogen receptors (ERs) through genomic action, but recently a membrane-bound G protein-coupled receptor, GPR30, was identified as a non-genomic estrogen receptor. In this study we investigated the presence and localization of GPR30 in the rat spinal cord and dorsal root ganglion (DRG) in comparison with ERα. Using immunohistochemistry and in situ hybridization, we showed the expression of GPR30 in DRG neurons in male and female rats at mRNA and protein levels without specific sexual difference. A dense accumulation of GPR30 immunoreactivity was observed in the outer layer of the spinal dorsal horn, and selective spinal dorsal rhizotomy revealed that GPR30 was transported from the DRG to terminals located in the spinal dorsal horn. GPR30 expression was downregulated in DRG neurons of ovariectomized female rats. The spinal somatosensory system might be modulated by estradiol via putative membrane ER, GPR30-mediated mechanism.
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影响因子：
2.7
作者：
Levin, ER
通讯作者：
Levin, ER
影响因子：
4
作者：
Mannino, Christy A.;South, Samantha M.;Inturrisi, Charles E.
通讯作者：
Inturrisi, Charles E.
影响因子：
7.4
作者：
Amandusson, Å;Hallbeck, M;Blomqvist, A
通讯作者：
Blomqvist, A
影响因子：
3.4
作者：
Hucho, Tim B.;Dina, Olayinka A.;Levine, Jon D.
通讯作者：
Levine, Jon D.
DOI：
10.1111/j.1468-2982.2008.01755.x
发表时间：
2009-05
期刊：
Cephalalgia : an international journal of headache
影响因子：
--
作者：
Liverman CS;Brown JW;Sandhir R;Klein RM;McCarson K;Berman NE
通讯作者：
Berman NE