An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.
An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.
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DOI：
10.1038/s41467-018-03142-x
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发表时间：
2018-02-22
影响因子：
16.6
通讯作者：
McQueen-Mason SJ
中科院分区：
文献类型：
--
作者：
Sabbadin F;Hemsworth GR;Ciano L;Henrissat B;Dupree P;Tryfona T;Marques RDS;Sweeney ST;Besser K;Elias L;Pesante G;Li Y;Dowle AA;Bates R;Gomez LD;Simister R;Davies GJ;Walton PH;Bruce NC;McQueen-Mason SJ
Thermobia astritica属于一个古老的昆虫类群，在没有微生物帮助的情况下具有消化结晶纤维素的显著能力。通过研究热菌的消化蛋白质组，我们已经确定了20多个成员的溶解性多糖单加氧酶（LPMO）的一个未知的家庭。我们表明，这个LPMO家族跨越生命之树的多个分支，具有古老的起源，并被早期节肢动物招募，可能在发育和变态过程中重塑内源性甲壳素支架中发挥作用。基于我们深入表征的Thermobia的LPMOs，我们提出，这些酶对纤维素消化的多样化可能赋予祖先昆虫生物质降解的有效生化装置，允许在古生代早期殖民化的土地。LPMO在现代农业害虫和疾病媒介中的重要作用为帮助应对粮食安全和传染病控制方面的全球挑战提供了新的机会。LPMO催化多糖的氧化分解，从而促进生物质降解。通过分析firebrats的消化蛋白质组，作者在这里确定了一个尚未表征的LPMO家族，并提供了系统发育，结构和生化的见解，其起源和功能。
Thermobia domestica belongs to an ancient group of insects and has a remarkable ability to digest crystalline cellulose without microbial assistance. By investigating the digestive proteome of Thermobia, we have identified over 20 members of an uncharacterized family of lytic polysaccharide monooxygenases (LPMOs). We show that this LPMO family spans across several clades of the Tree of Life, is of ancient origin, and was recruited by early arthropods with possible roles in remodeling endogenous chitin scaffolds during development and metamorphosis. Based on our in-depth characterization of Thermobia’s LPMOs, we propose that diversification of these enzymes toward cellulose digestion might have endowed ancestral insects with an effective biochemical apparatus for biomass degradation, allowing the early colonization of land during the Paleozoic Era. The vital role of LPMOs in modern agricultural pests and disease vectors offers new opportunities to help tackle global challenges in food security and the control of infectious diseases. LPMOs catalyze the oxidative breakdown of polysaccharides, thereby facilitating biomass degradation. By analyzing the digestive proteome of firebrats, the authors here identify a yet uncharacterized LPMO family and provide phylogenetic, structural and biochemical insights into its origin and functions.
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DOI：
10.1073/pnas.89.22.10915
发表时间：
1992-11-15
影响因子：
11.1
作者：
HENIKOFF, S;HENIKOFF, JG
通讯作者：
HENIKOFF, JG
影响因子：
15
作者：
Hemsworth, Glyn R.;Taylor, Edward J.;Kim, Robbert Q.;Gregory, Rebecca C.;Lewis, Sally J.;Turkenburg, Johan P.;Parkin, Alison;Davies, Gideon J.;Walton, Paul H.
通讯作者：
Walton, Paul H.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
14.9
作者：
Davis IW;Leaver-Fay A;Chen VB;Block JN;Kapral GJ;Wang X;Murray LW;Arendall WB 3rd;Snoeyink J;Richardson JS;Richardson DC
通讯作者：
Richardson DC
影响因子：
4.4
作者：
Dowle, Adam A.;Wilson, Julie;Thomas, Jerry R.
通讯作者：
Thomas, Jerry R.