Comparative Transcriptome Analysis of Genes Involved in Anthocyanin Biosynthesis in Red and Green Walnut (Juglans regia L.).
Comparative Transcriptome Analysis of Genes Involved in Anthocyanin Biosynthesis in Red and Green Walnut (Juglans regia L.).
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DOI：
10.3390/molecules23010025
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发表时间：
2017-12-22
期刊：
影响因子：
--
通讯作者：
Wu G
中科院分区：
文献类型：
--
作者：
Li Y;Luo X;Wu C;Cao S;Zhou Y;Jie B;Cao Y;Meng H;Wu G
果实颜色是一项重要的经济性状。红核桃品种的颜色主要归因于花青素。本研究旨在探讨红色核桃和绿色核桃叶片和果皮颜色变化的分子机制差异。对核桃的一个参考转录组进行了测序和注释，以寻找与成熟阶段果实颜色相关的基因。使用组合组装策略，超过2.9亿个高质量读数被组装成39,411个基因。利用Illumina数字基因表达谱技术，我们鉴定了成熟阶段红、绿核桃叶片差异表达基因4568个，红、绿核桃果皮差异表达基因3038个。我们还鉴定了一些参与花青素生物合成调控的转录因子家族(MYB、bHLH和WD40)。通过实时定量聚合酶链式反应，验证了红核桃和青核桃叶片和果皮中几个花青素生物合成酶和转录因子基因表达水平的变化趋势。总之，我们的结果确定了与红核桃花青素积累有关的基因。这些数据为了解红核桃的变色提供了有价值的资源。
Fruit color is an important economic trait. The color of red walnut cultivars is mainly attributed to anthocyanins. The aim of this study was to explore the differences in the molecular mechanism of leaf and peel color change between red and green walnut. A reference transcriptome of walnut was sequenced and annotated to identify genes related to fruit color at the ripening stage. More than 290 million high-quality reads were assembled into 39,411 genes using a combined assembly strategy. Using Illumina digital gene expression profiling, we identified 4568 differentially expressed genes (DEGs) between red and green walnut leaf and 3038 DEGs between red and green walnut peel at the ripening stage. We also identified some transcription factor families (MYB, bHLH, and WD40) involved in the control of anthocyanin biosynthesis. The trends in the expression levels of several genes encoding anthocyanin biosynthetic enzymes and transcription factors in the leaf and peel of red and green walnut were verified by quantitative real-time PCR. Together, our results identified the genes involved in anthocyanin accumulation in red walnut. These data provide a valuable resource for understanding the coloration of red walnut.
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DOI：
10.1111/j.1365-313x.2006.02964.x
发表时间：
2007-02
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Espley RV;Hellens RP;Putterill J;Stevenson DE;Kutty-Amma S;Allan AC
通讯作者：
Allan AC
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
7.4
作者：
Griesser, Markus;Hoffmann, Thomas;Schwab, Wilfried
通讯作者：
Schwab, Wilfried
影响因子：
11.6
作者：
Albert, Nick W.;Davies, Kevin M.;Schwinn, Kathy E.
通讯作者：
Schwinn, Kathy E.
影响因子：
7.4
作者：
Huang, Junli;Gu, Min;Chen, Zhixiang
通讯作者：
Chen, Zhixiang