Novel Intraperitoneal Treatment With Non-Thermal Plasma-Activated Medium Inhibits Metastatic Potential of Ovarian Cancer Cells.
Novel Intraperitoneal Treatment With Non-Thermal Plasma-Activated Medium Inhibits Metastatic Potential of Ovarian Cancer Cells.
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使用非热等离子体激活介质的新型腹腔内治疗可抑制卵巢癌细胞的转移潜力。
DOI：
10.1038/s41598-017-05620-6
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发表时间：
2017-07-20
影响因子：
4.6
通讯作者：
Kajiyama H
中科院分区：
文献类型：
--
作者：
Nakamura K;Peng Y;Utsumi F;Tanaka H;Mizuno M;Toyokuni S;Hori M;Kikkawa F;Kajiyama H
非热大气压等离子体已被提出作为一种新的治疗癌症的工具。近年来，等离子体活化介质（PAM）在各种癌症类型中的应用得到了广泛的研究。然而，在反映病理情况的动物模型中证实PAM的抗肿瘤作用及其机制的报道很少。我们在体外和体内研究了PAM对卵巢癌ES2细胞转移的抑制作用。我们证明，在一定的PAM稀释比例下，PAM可以抑制ES2细胞的迁移、侵袭和粘附，而细胞活力不受影响。在腹膜内转移的小鼠体内模型中，PAM抑制ES2细胞的腹膜播散，从而延长了存活时间。此外，我们评估了分子机制，发现PAM降低了MMP-9。在进一步的研究中，我们还发现PAM通过抑制JNK1/2和p38 MAPK的磷酸化来阻止MAPK通路的激活。这些发现表明，PAM通过减少对癌细胞运动至关重要的MMP-9分泌来抑制卵巢癌细胞的转移。我们的研究结果表明，PAM腹腔内治疗可能是卵巢癌的一种有希望的治疗选择。
Non-thermal atmospheric pressure plasma has been proposed as a new therapeutic tool for cancer treatment. Recently, plasma-activated medium (PAM) has been widely studied in various cancer types. However, there are only few reports demonstrating the anti-tumour effects of PAM in an animal model reflecting pathological conditions and the accompanying mechanism. Here we investigated the inhibitory effect of PAM on the metastasis of ovarian cancer ES2 cells in vitro and in vivo. We demonstrated that ES2 cell migration, invasion and adhesion were suppressed by PAM at a certain PAM dilution ratio, whereas cell viability remained unaffected. In an in vivo mouse model of intraperitoneal metastasis, PAM inhibited peritoneal dissemination of ES2 cells, resulting in prolonged survival. Moreover, we assessed the molecular mechanism and found that MMP-9 was decreased by PAM. On further investigation, we also found that PAM prevented the activation of the MAPK pathway by inhibiting the phosphorylation of JNK1/2 and p38 MAPK. These findings indicate that PAM inhibits the metastasis of ovarian cancer cells through reduction of MMP-9 secretion, which is critical for cancer cell motility. Our findings suggest that PAM intraperitoneal therapy may be a promising treatment option for ovarian cancer.
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DOI：
10.1038/nrc2644
发表时间：
2009-06
期刊：
Nature reviews. Cancer
影响因子：
--
作者：
通讯作者：
--
影响因子：
4
作者：
Iseki, Sachiko;Nakamura, Kae;Hori, Masaru
通讯作者：
Hori, Masaru
影响因子：
3.6
作者：
Kajiyama, Hiroaki;Shibata, Kiyosumi;Kikkawa, Fumitaka
通讯作者：
Kikkawa, Fumitaka
影响因子：
1.5
作者：
Kajiyama, Hiroaki;Nakamura, Kae;Kikkawa, Fumitaka
通讯作者：
Kikkawa, Fumitaka
DOI：
10.3322/caac.20113
发表时间：
2011-05
期刊：
CA: a cancer journal for clinicians
影响因子：
--
作者：
Jelovac D;Armstrong DK
通讯作者：
Armstrong DK