C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats.
C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats.
复制标题
C反应蛋白敲除可减轻大鼠颞下颌关节炎症
DOI：
10.1155/2022/8613986
复制
发表时间：
2022
影响因子：
4.1
通讯作者：
Song J
中科院分区：
文献类型：
--
作者：
He Y;Zhou M;Jian Z;Fang L;Huang L;Song J
C-反应蛋白（CRP）是炎症的生物标志物，在骨关节炎（OA）相关疾病中高度表达，但其确切作用尚不清楚。在这项研究中，我们评估了CRP对颞下颌关节（TMJ）炎症的生物学效应。 使用弗氏完全佐剂（CFA）在CRP敲除（CRP-/-）和对照大鼠中诱导TMJ炎症。比较TMJ的退行性变化，以阐明CRP在TMJ炎症中的作用。此外，通过实时定量聚合酶链反应、免疫组织化学和抗酒石酸盐磷酸酶染色评价炎性细胞因子、巨噬细胞活化和破骨细胞分化，以探索潜在的调控机制。 与对照组相比，CFA诱导TMJ炎症，增加全身和局部CRP表达。此外，CRP-/-大鼠表现出不太严重的炎症症状。在CRP-/-大鼠中检测到促炎细胞因子（白细胞介素-（IL-）1β和IL-6）的下调和抗炎细胞因子IL-10的上调，这也表现出减少的巨噬细胞活化和破骨细胞分化。 这些结果表明，控制炎症过程中CRP的高度升高水平可以改变细胞因子谱、巨噬细胞活化和破骨细胞分化，从而为TMJ-OA的预防和治疗提供有益的效果。
C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis- (OA-) related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation. Freund's complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. In addition, inflammatory cytokines, macrophage activation, and osteoclast differentiation were evaluated by real-time quantitative polymerase chain reaction, immunohistochemistry, and tartrate-resistant phosphatase staining to explore the potential regulatory mechanism. Compared to the control, CFA induced TMJ inflammation, which increased systemic and local CRP expression. Furthermore, CRP-/- rats exhibited less severe inflammatory symptoms. The downregulation of proinflammatory cytokines (interleukin- (IL-) 1β and IL-6) and upregulation of the anti-inflammatory cytokine IL-10 were detected in CRP-/- rats, which also exhibited reduced macrophage activation and osteoclast differentiation. These results indicated that controlling the highly elevated levels of CRP during inflammation could modify the cytokine profile, macrophage activation, and osteoclast differentiation, thus, providing beneficial effects for TMJ-OA prevention and treatment.
登录
查看更多内容
CRP结构的过渡变化导致促炎结合位点的暴露。
影响因子：
16.6
作者：
Braig D;Nero TL;Koch HG;Kaiser B;Wang X;Thiele JR;Morton CJ;Zeller J;Kiefer J;Potempa LA;Mellett NA;Miles LA;Du XJ;Meikle PJ;Huber-Lang M;Stark GB;Parker MW;Peter K;Eisenhardt SU
通讯作者：
Eisenhardt SU
影响因子：
4.4
作者：
Devaraj S;Singh U;Jialal I
通讯作者：
Jialal I
单体 C 反应蛋白结合并中和 NF-κB 配体诱导的破骨细胞分化的受体激活剂
影响因子：
7.3
作者：
Jia ZK;Li HY;Liang YL;Potempa LA;Ji SR;Wu Y
通讯作者：
Wu Y
影响因子：
1.9
作者：
Gunson, Michael J.;Arnett, G. William;Milam, Stephen B.
通讯作者：
Milam, Stephen B.
影响因子：
0.8
作者：
Fuentes, R.;Veuthey, C.;Ottone, N. E.
通讯作者：
Ottone, N. E.