Enhancement of human immunodeficiency virus type 1 infection by antisera to peptides from the envelope glycoproteins gp120/gp41.
Enhancement of human immunodeficiency virus type 1 infection by antisera to peptides from the envelope glycoproteins gp120/gp41.
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包膜糖蛋白 gp120/gp41 肽的抗血清增强人类免疫缺陷病毒 1 型感染。
DOI：
10.1084/jem.174.6.1557
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发表时间：
1991-12-01
期刊：
影响因子：
--
通讯作者：
Neurath AR
中科院分区：
文献类型：
--
作者：
Jiang SB;Lin K;Neurath AR
人类免疫缺陷病毒1型（HIV-1）包膜糖蛋白（gp 120和gp 41）可引发病毒中和抗体（VNAB）以及增强HIV-1感染的抗体（EAB）。已经定义了几个引起VNAB的表位，主要的病毒中和决定簇被分配到gp 120的V3环。为了提供一个合理设计抗HIV疫苗的背景，它似乎也很重要，以确定域引发EAB。这是通过筛选抗合成肽的抗血清来实现的，所述合成肽几乎覆盖gp 120/gp 41的整个序列，以增强MT-2细胞（一种连续的T细胞系）对HIV-1感染的作用。许多（16/30）的抗血清显着增强HIV-1在人补体的存在下。补体受体2型（CR2）抗体消除了抗体介导的HIV-1感染增强作用。还测试了21种不同HIV-1分离株的V3高变环抗血清对HIV-1 IIIB感染的增强作用。这些血清中有11份含有VNAB，10份含有增强的HIV-1 IIIB感染。所有具有病毒增强活性的抗血清都含有与HIV-1 IIIB的V3环交叉反应的抗体，并且病毒增强活性随着血清学交叉反应性的增加而增加。这些结果表明，如果免疫原上的表位和感染人群的主要HIV-1分离株不充分匹配，即，血清学交叉反应，但在病毒中和水平上不交叉反应。
Human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (gp120 and gp41) elicit virus-neutralizing antibodies (VNAB) and also antibodies enhancing HIV-1 infection (EAB). Several epitopes eliciting VNAB have been defined, the principal virus-neutralizing determinant being assigned to the V3 loop of gp120. To provide a background for a rational design of anti-HIV vaccines, it also appears important to define domains eliciting EAB. This was accomplished by screening antisera against synthetic peptides covering almost the entire sequence of gp120/gp41 for their enhancing effects on HIV-1 infection of MT-2 cells, a continuous T cell line. Many (16/30) of the antisera significantly enhanced HIV-1 in the presence of human complement. Antibodies to complement receptor type 2 (CR2) abrogated the antibody- mediated enhancement of HIV-1 infection. Antisera to V3 hypervariable loops of 21 distinct HIV-1 isolates were also tested for their enhancing effects on HIV-1IIIB infection. 11 of these sera contained VNAB and 10 enhanced HIV-1IIIB infection. All antisera with virus- enhancing activity contained antibodies crossreactive with the V3 loop of HIV-1IIIB, and the virus-enhancing activity increased with increasing serological crossreactivity. These results suggest that immunization with antigens encompassing V3 loops may elicit EAB rather than protective antibodies if epitopes on the immunogen and the predominant HIV-1 isolate infecting a population are insufficiently matched, i.e., crossreactive serologically but not at the level of virus neutralization.
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影响因子：
39.2
作者：
DOLIN, R;GRAHAM, BS;KOFF, WC
通讯作者：
KOFF, WC
影响因子：
15.3
作者：
JIANG, S;PERSECHINI, PM;YOUNG, JDE
通讯作者：
YOUNG, JDE
影响因子：
56.9
作者：
LASKY, LA;GROOPMAN, JE;BERMAN, PW
通讯作者：
BERMAN, PW
影响因子：
6.4
作者：
GNANN, JW;SCHWIMMBECK, PL;OLDSTONE, MBA
通讯作者：
OLDSTONE, MBA
影响因子：
5.4
作者：
NARA, PL;SMIT, L;GOUDSMIT, J
通讯作者：
GOUDSMIT, J