QTL mapping of male sterility and transmission pattern in progeny of Satsuma mandarin.
QTL mapping of male sterility and transmission pattern in progeny of Satsuma mandarin.
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DOI：
10.1371/journal.pone.0200844
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Shimizu T
中科院分区：
文献类型：
--
作者：
Goto S;Yoshioka T;Ohta S;Kita M;Hamada H;Shimizu T
无核是柑橘育种的重要性状之一。温州蜜柑的雄性不育在日本柑橘育种中已被用于获得无核品种。开发与无核性状连锁的选择标记将有助于提高无核品种的育种效率。本研究以“冲津46号”ד冲津56号”（O 46-O 56）杂交组合为材料，对作为雄性不育指标的每花药花粉粒数（NPG）和花粉育性（APF）进行了QTL定位，在第8连锁群上检测到一个NPG候选QTL（MS-P1），LOD值为7.31，可解释47%的方差。在第6连锁群上检测到APF（MS-F1）QTL，LOD值为5.71，解释方差的63.6%。用'冲津46号' × '卡拉'（O 46-K）杂交证实了MS-P1和MS-F1对NPG和APF的降低作用。系谱分析表明，“兴津46号”的MS-P1和MS-F1均来源于柑桔（Citrus nobilis）的久延宝（Kunenbo）经八朔（C.《甜蜜的春天》（Sweet Spring）细胞质分析表明，雄性不育系‘甜春’和‘兴津46号’的细胞质来源于纪州（C。kunenbo和hassaku的细胞质来源于其他品种而不是Kishu。这些结果表明，MS-P1和MS-F1主要减少NPG和减少APF，但它们的表达需要来自Kishu的细胞质。这些结果将有助于我们进一步了解柑橘雄性不育的分子机制，并有助于开发柑橘无核育种的DNA标记。
Seedlessness is one of the important traits in citrus breeding. Male sterility derived from Satsuma mandarin (Citrus unshiu) has been used in Japanese citrus breeding programs to obtain seedless cultivars. The efficiency of seedless cultivar breeding would be improved by developing a selection marker linked to seedlessness. In this study, we performed QTL mapping in ‘Okitsu No. 46’ × ‘Okitsu No. 56’ (O46-O56) crosses for the number of pollen grains per anther (NPG) and apparent pollen fertility (APF), two traits used as an index of male sterility, and detected a candidate QTL for NPG (MS-P1) on linkage group 8 with a significant LOD score (7.31) and 47% of variance explained. The QTL for APF (MS-F1) was detected on linkage group 6 with a significant LOD score (5.71) and 63.6% of variance explained. The role of both MS-P1 in reducing NPG and MS-F1 in decreasing APF were confirmed with the ‘Okitsu No.46’ × ‘Kara’ (O46-K) cross. Pedigree analysis inferred that both MS-P1 and MS-F1 in ‘Okitsu No. 46’ were derived from kunenbo (Citrus nobilis) through hassaku (C. hassaku) and ‘Sweet Spring’. Cytoplasm analysis revealed that both male-sterile ‘Sweet Spring’ and ‘Okitsu No. 46’ have cytoplasm derived from Kishu (C. kinokuni hort. ex Tanaka), but the cytoplasm of male-sterile kunenbo and hassaku were derived from other varieties rather than Kishu. These results suggest that MS-P1 and MS-F1 primarily reduce the NPG and decrease APF, but their expression requires a cytoplasm derived from Kishu. These findings will improve our understanding of the molecular mechanism of male sterility in citrus and help to develop a DNA marker for seedless breeding in citrus.
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核和细胞质标志物揭示的父母诊断Satsuma普通话（柑橘类citrus unshiu Marc。）。
影响因子：
2.4
作者：
Fujii H;Ohta S;Nonaka K;Katayose Y;Matsumoto T;Endo T;Yoshioka T;Omura M;Shimada T
通讯作者：
Shimada T
影响因子：
3
作者：
Cai, Xiao-Dong;Fu, Jing;Guo, Wen-Wu
通讯作者：
Guo, Wen-Wu
影响因子：
3.7
作者：
Shimizu T;Kitajima A;Nonaka K;Yoshioka T;Ohta S;Goto S;Toyoda A;Fujiyama A;Mochizuki T;Nagasaki H;Kaminuma E;Nakamura Y
通讯作者：
Nakamura Y
影响因子：
2.7
作者：
SHAPIRO, SS;WILK, MB
通讯作者：
WILK, MB
影响因子：
3
作者：
Ollitrault, Frederique;Terol, Javier;Ollitrault, Patrick
通讯作者：
Ollitrault, Patrick