Influenza virus in human exhaled breath: an observational study.
Influenza virus in human exhaled breath: an observational study.
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DOI：
10.1371/journal.pone.0002691
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发表时间：
2008-07-16
期刊：
影响因子：
3.7
通讯作者：
Milton, Donald K.
中科院分区：
文献类型：
--
作者：
Fabian, Patricia;McDevitt, James J.;DeHaan, Wesley H.;Fung, Rita O. P.;Cowling, Benjamin J.;Chan, Kwok Hung;Leung, Gabriel M.;Milton, Donald K.
最近的研究表明，人类在潮汐呼吸过程中呼出细颗粒，但对其成分知之甚少，特别是在感染期间。我们对流感感染患者进行了一项研究，以表征他们呼出气中的流感病毒和颗粒浓度。在中国香港的三家诊所招募了表现为流感样疾病、经快速检测确诊为甲型或B型流感病毒且在3天内发病的患者。我们收集每个受试者的呼出气到聚四氟乙烯过滤器，并使用光学颗粒计数器测量呼出的颗粒浓度。通过定量聚合酶链反应（qPCR）分析过滤器中的甲型和B型流感病毒。13例快速检测阳性患者中有12例提供了呼出气过滤器样本（7例受试者感染B型流感病毒，5例受试者感染A型流感病毒）。我们在4名（33%）受试者的呼出气中检测到流感病毒RNA，其中5名感染甲型流感病毒的患者中有3名（60%），7名感染B型流感病毒的患者中有1名（14%）。呼出的流感病毒RNA产生速率范围为每分钟<3.2至20个流感病毒RNA颗粒。超过87%的呼出颗粒直径小于1微米。这些关于流感病毒RNA的发现表明，流感病毒可能包含在潮汐呼吸过程中产生的细颗粒中，并增加了表明细颗粒气溶胶可能在流感传播中发挥作用的文献。
Recent studies suggest that humans exhale fine particles during tidal breathing but little is known of their composition, particularly during infection. We conducted a study of influenza infected patients to characterize influenza virus and particle concentrations in their exhaled breath. Patients presenting with influenza-like-illness, confirmed influenza A or B virus by rapid test, and onset within 3 days were recruited at three clinics in Hong Kong, China. We collected exhaled breath from each subject onto Teflon filters and measured exhaled particle concentrations using an optical particle counter. Filters were analyzed for influenza A and B viruses by quantitative polymerase chain reaction (qPCR). Twelve out of thirteen rapid test positive patients provided exhaled breath filter samples (7 subjects infected with influenza B virus and 5 subjects infected with influenza A virus). We detected influenza virus RNA in the exhaled breath of 4 (33%) subjects–three (60%) of the five patients infected with influenza A virus and one (14%) of the seven infected with influenza B virus. Exhaled influenza virus RNA generation rates ranged from <3.2 to 20 influenza virus RNA particles per minute. Over 87% of particles exhaled were under 1 µm in diameter. These findings regarding influenza virus RNA suggest that influenza virus may be contained in fine particles generated during tidal breathing, and add to the body of literature suggesting that fine particle aerosols may play a role in influenza transmission.
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影响因子：
--
作者：
Edward, DGF;Elford, WJ;Laidlaw, PP
通讯作者：
Laidlaw, PP
DOI：
10.1073/pnas.0408159101
发表时间：
2004-12-14
影响因子：
11.1
作者：
Edwards, DA;Man, JC;Scheuch, G
通讯作者：
Scheuch, G
影响因子：
5
作者：
RILEY, EC;MURPHY, G;RILEY, RL
通讯作者：
RILEY, RL
影响因子：
3.7
作者：
Cowling BJ;Fung RO;Cheng CK;Fang VJ;Chan KH;Seto WH;Yung R;Chiu B;Lee P;Uyeki TM;Houck PM;Peiris JS;Leung GM
通讯作者：
Leung GM
DOI：
10.1089/jam.1997.10.105
发表时间：
1997-01-01
期刊：
Journal of aerosol medicine : the official journal of the International Society for Aerosols in Medicine
影响因子：
--
作者：
Papineni, R S;Rosenthal, F S
通讯作者：
Rosenthal, F S