Analysis of Aspergillus nidulans conidial antigens and their prevalence in other Aspergillus species
Analysis of Aspergillus nidulans conidial antigens and their prevalence in other Aspergillus species
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构巢曲霉分生孢子抗原及其在其他曲霉属物种中的流行率分析
DOI：
10.1128/iai.59.12.4478-4485.1991
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发表时间：
1991
影响因子：
3.1
通讯作者：
F. Leal
中科院分区：
文献类型：
--
作者：
P. Puente;M. Ovejero;Nola Fernández;F. Leal
Nidulans曲霉是一种子囊菌，通过形成多细胞分生孢子和称为分生孢子的单核孢子进行无性繁殖。这些成分构成了这种致病曲霉和其他致病曲霉的主要传播媒介，也是不同曲霉病的起点。为了利用假单胞菌作为免疫诊断有用抗原的潜在来源，我们用十二烷基硫酸钠(十二烷基硫酸钠)-聚丙烯酰胺凝胶电泳法测定了该真菌分生孢子提取物在不同比例凝胶中的总蛋白组成。其中几种免疫原既存在于菌丝提取物中，又被抗菌丝体抗体识别。在其他病原性曲霉菌的分生孢子提取液中也发现了其中的4种，分别命名为CDA、CDB、CDC和CDE。在无亲缘关系的镰刀菌和黑点霉菌的细胞提取液中仅检测不到CDE。
Aspergillus nidulans is an ascomycetous fungus that reproduces asexually by forming multicellular conidiophores and uninucleate spores called conidia. These elements constitute the main vehicle for the transmission of this and other pathogenic Aspergillus species and are the starting point of the different forms of aspergillosis. In order to use A. nidulans as a potential source of useful antigens for the immunodiagnosis of these diseases, we have examined the total protein composition of conidial extracts of this fungus by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis in gels of different percent T. Injection of SDS-extracted conidial proteins into rabbits allowed us to raise a battery of polyclonal antibodies which have defined some important immunogenic polypeptides. Several of these immunogens were both present in mycelial extracts and recognized by antimycelium antibodies. Four of them, designated cdA, cdB, cdC, and cdE, were also found in conidial extracts of other pathogenic Aspergillus species. Only cdE was undetectable in cell extracts of the nonrelated species Fusarium culmorum and Phycomyces blakesleeanus.
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影响因子：
4.6
作者：
Piechura,JE;Kurup,VP;Fink,JN;Calvanico,NJ
通讯作者：
Calvanico,NJ
DOI：
10.1080/02681218780000581
发表时间：
1987-07
期刊：
Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology
影响因子：
--
作者：
J. Piechura;R. S. Riefel;L. Daft
通讯作者：
J. Piechura;R. S. Riefel;L. Daft
烟曲霉抗原的酶谱和免疫化学特征。
DOI：
10.1016/0091-6749(86)90267-8
发表时间：
1986
期刊：
The Journal of allergy and clinical immunology
影响因子：
--
作者：
Kurup,VP;Resnick,A;Scribner,GH;Gunasekaran,M;Fink,JN
通讯作者：
Fink,JN
来自烟曲霉的部分纯化的糖蛋白抗原。
DOI：
10.1159/000233984
发表时间：
1986
期刊：
International archives of allergy and applied immunology
影响因子：
--
作者：
Kurup,VP;John,KV;Resnick,A;Fink,JN
通讯作者：
Fink,JN