Insights into the Draft Genome Sequence of the Kiwifruit-Associated Pathogenic Isolate Pseudomonas fluorescens AHK-1

Insights into the Draft Genome Sequence of the Kiwifruit-Associated Pathogenic Isolate Pseudomonas fluorescens AHK-1
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猕猴桃相关致病分离株荧光假单胞菌 AHK-1 基因组序列草案的见解

DOI:
10.1007/s00284-019-01655-2
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发表时间:
2019-03
影响因子:
2.6
通讯作者:
Song J H
Song J H
中科院分区:
生物学4区
文献类型:
--
作者:
Zhang L X;Wu Z R;Wang X;Tan G J;Song J H

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荧光假单胞菌是存在于许多生境中的生理上多样的细菌物种,其具有多功能特性,使其具有表现出生物控制活性、促进植物健康或引起植物疾病的能力。在这里,我们提出了猕猴桃相关的病原分离物AHK-1的荧光假单胞菌,这是从猕猴桃植物的病叶分离的基因组序列草案。AHK-1基因组大小为7,035,786 bp,G + C含量为60.88%。根据比较基因组分析,预测其总共包含6327个基因,其中3998个与另外两个测序的荧光假单胞菌分离株(SBW 25和GcM 5 -1A)中的基因同源,946个是AHK-1特有的。此外,我们确定了AHK-1基因组中的几个候选毒力因子,包括编码鞭毛生物合成蛋白的fliA基因,以及VI、III和IV型分泌系统的基因。该基因组资源将作为更好地理解致病性和非致病性菌株的遗传学的参考,并将有助于阐明与植物病害相关的荧光假单胞菌的致病机制。
Pseudomonas fluorescens is a physiologically diverse species of bacteria present in many habitats, which possesses multifunctional traits that provide it with the capability to exhibit biological control activities, promote plant health or cause plant disease. Here, we present the draft genome sequence of the kiwifruit-associated pathogenic isolate AHK-1 of P. fluorescens, which was isolated from the diseased leaves of kiwifruit plants. The genome size of AHK-1 was found to be 7,035,786 bp, with a G + C content of 60.88%. It is predicted to contain a total of 6327 genes, of which 3998 were homologous to genes in the other two sequenced P. fluorescens isolates (SBW25 and GcM5-1A) and 946 were unique to AHK-1 based on comparative genomic analysis. Furthermore, we identified several candidate virulence factors in the genome of AHK-1, including the fliA gene encoding flagellar biosynthetic protein for biosynthesis, and the genes for components of type VI, III, and IV secretion systems. This genomic resource will serve as a reference for better understanding the genetics of pathogenic and non-pathogenic strains, and will help to elucidate the pathogenic mechanisms of P. fluorescens associated with plant disease.
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