A reproducible oral microcosm biofilm model for testing dental materials.
A reproducible oral microcosm biofilm model for testing dental materials.
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DOI：
10.1111/j.1365-2672.2012.05439.x
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发表时间：
2012-12
影响因子：
4
通讯作者：
Aparicio C
中科院分区：
文献类型：
--
作者：
Rudney JD;Chen R;Lenton P;Li J;Li Y;Jones RS;Reilly C;Fok AS;Aparicio C
大多数关于生物膜对牙科材料影响的研究使用单一物种生物膜或联合体。直接从唾液或牙菌斑生长的微宇宙生物膜更加多样化，但难以表征。我们使用人类口腔微生物鉴定微阵列（HOMIM）来验证可重复的口腔微观世界模型。收集成人和儿童的唾液和牙菌斑。用唾液或菌斑接种羟基磷灰石和牙科复合材料盘，并在CDC生物膜反应器中生长微宇宙生物膜。在随后的实验中，反应器用蔗糖脉冲。对272个种的接种物和微宇宙的DNA进行了HOMIM分析。微宇宙包括来自原始接种物的约60%的物种。羟基磷灰石和复合材料上生长的生物膜非常相似。蔗糖脉冲降低的多样性和pH值，但增加了丰富的链球菌和韦荣氏球菌。来自同一供体的生物膜，在不同时间生长，聚集在一起。该模型产生了代表口腔微生物群的可再现的微宇宙生物膜。蔗糖诱导的变化与龋齿有关。这是首次使用HOMIM验证口腔微观模型，该模型可用于研究复杂生物膜对牙科材料的影响。
Most studies of biofilm effects on dental materials use single-species biofilms, or consortia. Microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. We used the Human Oral Microbial Identification Microarray (HOMIM) to validate a reproducible oral microcosm model. Saliva and dental plaque were collected from adults and children. Hydroxyapatite and dental composite disks were inoculated with either saliva or plaque, and microcosm biofilms were grown in a CDC biofilm reactor. In later experiments, the reactor was pulsed with sucrose. DNA from inoculums and microcosms were analyzed by HOMIM for 272 species. Microcosms included about 60% of species from the original inoculum. Biofilms grown on hydroxyapatite and composites were extremely similar. Sucrose-pulsing decreased diversity and pH, but increased the abundance of Streptococcus and Veilonella. Biofilms from the same donor, grown at different times, clustered together. This model produced reproducible microcosm biofilms that were representative of the oral microbiota. Sucrose induced changes associated with dental caries. This is the first use of HOMIM to validate an oral microcosm model that can be used to study the effects of complex biofilms on dental materials.
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影响因子：
5
作者：
Matharu, S;Spratt, DA;Gulabivala, K
通讯作者：
Gulabivala, K
影响因子：
4.2
作者：
Azevedo, M. S.;van de Sande, F. H.;Cenci, M. S.
通讯作者：
Cenci, M. S.
影响因子：
3.7
作者：
Ahn J;Yang L;Paster BJ;Ganly I;Morris L;Pei Z;Hayes RB
通讯作者：
Hayes RB
影响因子：
4
作者：
Ledder, R. G.;Gilbert, P.;McBain, A. J.
通讯作者：
McBain, A. J.
影响因子：
5
作者：
Beyth, Nurit;Bahir, Ran;Weiss, Ervin I.
通讯作者：
Weiss, Ervin I.