PHB Biosynthesis Counteracts Redox Stress in Herbaspirillum seropedicae.
PHB Biosynthesis Counteracts Redox Stress in Herbaspirillum seropedicae.
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DOI：
10.3389/fmicb.2018.00472
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发表时间：
2018
影响因子：
5.2
通讯作者：
Müller-Santos M
中科院分区：
文献类型：
--
作者：
Batista MB;Teixeira CS;Sfeir MZT;Alves LPS;Valdameri G;Pedrosa FO;Sassaki GL;Steffens MBR;de Souza EM;Dixon R;Müller-Santos M
细菌产生聚羟基链烷酸酯如聚（3-羟基丁酸酯）（PHB）的能力使得能够提供可以在苛刻的生理条件下被动员的碳储存分子。然而，PHB在细胞代谢中的确切功能尚未明确定义。为了确定PHB生产对全球生理的影响，我们已经表征了重氮营养细菌Herbaspirillum seropedicae的Δ phaC 1突变株的特性。在突变株中没有PHB不仅扰乱氧化还原平衡和增加氧化应激，而且还影响氧化还原敏感Fnr转录调节因子的活性，导致电子传递链的细胞色素c分支的表达发生显著变化。PHB的合成本身依赖于Fnr 1和Fnr 3蛋白，导致将PHB的合成与氧化还原调节偶联的循环依赖性。对Δ phaC 1突变体的转录谱分析表明，PHB合成的丧失影响了许多基因的表达，包括约30%的Fnr调节子。
The ability of bacteria to produce polyhydroxyalkanoates such as poly(3-hydroxybutyrate) (PHB) enables provision of a carbon storage molecule that can be mobilized under demanding physiological conditions. However, the precise function of PHB in cellular metabolism has not been clearly defined. In order to determine the impact of PHB production on global physiology, we have characterized the properties of a ΔphaC1 mutant strain of the diazotrophic bacterium Herbaspirillum seropedicae. The absence of PHB in the mutant strain not only perturbs redox balance and increases oxidative stress, but also influences the activity of the redox-sensing Fnr transcription regulators, resulting in significant changes in expression of the cytochrome c-branch of the electron transport chain. The synthesis of PHB is itself dependent on the Fnr1 and Fnr3 proteins resulting in a cyclic dependency that couples synthesis of PHB with redox regulation. Transcriptional profiling of the ΔphaC1 mutant reveals that the loss of PHB synthesis affects the expression of many genes, including approximately 30% of the Fnr regulon.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
4.6
作者：
Sacomboio ENM;Kim EYS;Ruchaud Correa HL;Bonato P;de Oliveira Pedrosa F;de Souza EM;Chubatsu LS;Müller-Santos M
通讯作者：
Müller-Santos M
影响因子：
14.8
作者：
Bennett, Bryson D.;Yuan, Jie;Rabinowitz, Joshua D.
通讯作者：
Rabinowitz, Joshua D.
DOI：
10.1073/pnas.0609514104
发表时间：
2007-02-13
影响因子：
11.1
作者：
Crack, Jason C.;Green, Jeffrey;Thomson, Andrew J.
通讯作者：
Thomson, Andrew J.
DOI：
10.1007/978-981-10-2555-6_2
发表时间：
2016-01-01
期刊：
MICROBIAL MODELS: FROM ENVIRONMENTAL TO INDUSTRIAL SUSTAINABILITY
影响因子：
--
作者：
Batista, Marcelo Bueno;Mueller-Santos, Marcelo;de Souza, Emanuel Maltempi
通讯作者：
de Souza, Emanuel Maltempi