Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production.
Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production.
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DOI：
10.1007/s11248-017-0025-2
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发表时间：
2017-08
影响因子：
3
通讯作者：
Harvey BK
中科院分区：
文献类型：
--
作者：
Hamra FK;Richie CT;Harvey BK
Long Evans大鼠品系被用作广泛生物医学领域的研究模型（> 15，800次引用，NCBI PubMed）。在这里，我们报告了一种方法，以遗传修饰的龙埃文斯大鼠生殖细胞供体精原干细胞。Long Evans大鼠精原细胞系来源于新鲜分离的层粘连蛋白结合精原细胞。层粘连蛋白结合的精原细胞在含有GDNF和FGF 2的无血清培养基中的成纤维细胞饲养层上培养多代。通过转座子转导对Long Evans大鼠精原细胞系进行遗传修饰，以表达种系tdTomato报告基因。供体大鼠精原细胞系移植到白消安处理的同种异体Long Evans大鼠睾丸后，可稳健再生精子发生。供体来源的精子发生在很大程度上恢复了化学绝育的受体Long Evans大鼠的睾丸大小。Long-Evans大鼠稳定地将tdTomato生殖系标记传递给后代。总之，Long Evans大鼠精原细胞系为遗传修饰Long Evans大鼠提供了有效的供体生殖系载体。
Long Evans rat strains are applied as research models in a broad spectrum of biomedical fields (>15,800 citations, NCBI PubMed). Here, we report an approach to genetically modify the Long Evans rat germline in donor spermatogonial stem cells. Long Evans rat spermatogonial lines were derived from freshly isolated laminin-binding spermatogonia. Laminin-binding spermatogonia were cultured over multiple passages on fibroblast feeder layers in serum-free culture medium containing GDNF and FGF2. Long Evans rat spermatogonial lines were genetically modified by transposon transduction to express a germline, tdTomato reporter gene. Donor rat spermatogonial lines robustly regenerated spermatogenesis after transplantation into testes of busulfan-treated, allogenic, Long Evans rats. Donor-derived spermatogenesis largely restored testis size in the chemically sterilized, recipient Long Evans rats. Recipient Long Evans rats stably transmitted the tdTomato germline marker to subsequent generations. Overall, Long Evans rat spermatogonial lines provided effective donor germline vectors for genetically modifying Long Evans rats.
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影响因子：
9.5
作者：
Laulederkind SJ;Hayman GT;Wang SJ;Smith JR;Lowry TF;Nigam R;Petri V;de Pons J;Dwinell MR;Shimoyama M;Munzenmaier DH;Worthey EA;Jacob HJ
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3.6
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影响因子：
3.3
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通讯作者：
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影响因子：
4.8
作者：
Katter, Katharina;Geurts, Aron M.;Izsvak, Zsuzsanna
通讯作者：
Izsvak, Zsuzsanna