Nanotexture Optimization by Oxygen Plasma of Mesoporous Silica Thin Film for Enrichment of Low Molecular Weight Peptides Captured from Human Serum.
Nanotexture Optimization by Oxygen Plasma of Mesoporous Silica Thin Film for Enrichment of Low Molecular Weight Peptides Captured from Human Serum.
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DOI：
10.1007/s11426-010-4121-x
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发表时间：
2010-11-01
影响因子：
9.6
通讯作者：
Ferrari, Mauro
中科院分区：
文献类型：
--
作者：
Hu Ye;Peng Yang;Brousseau, Louis;Bouamrani, Ali;Liu XueWu;Ferrari, Mauro
本研究以氧等离子体与热氧化法来探讨介孔氧化矽薄膜之最佳化。在氧等离子体中的煅烧提供了对孔形成的关于孔表面的上级控制和对聚合物模板结构的更高保真度。所得的多孔膜提供了一个理想的基板从复杂的混合物中选择性分配的肽。利用XRD、椭偏仪、FTIR、TEM和N2吸附/脱附等手段对多孔薄膜的孔径分布、纳米结构、表面性质和孔连通性等物理化学性能进行了系统的表征。通过比较两种方法制备的介孔二氧化硅薄膜的MALDI-TOF MS谱，研究了从人血清中捕获的低分子量蛋白质在介孔二氧化硅薄膜上的富集。这种新型的芯片上分离技术提供了从人血清中回收低分子量肽的优势，这已被公认为癌症和其他疾病的早期诊断的信息资源。
This study investigated the optimization of mesoporous silica thin films by nanotexturing using oxygen plasma versus thermal oxidation. Calcination in oxygen plasma provides superior control over pore formation with regard to the pore surface and higher fidelity to the structure of the polymer template. The resulting porous film offers an ideal substrate for the selective partitioning of peptides from complex mixtures. The improved chemico-physical characteristics of porous thin films (pore size distribution, nanostructure, surface properties and pore connectivity) were systematically characterized with XRD, Ellipsometry, FTIR, TEM and N2 adsorption/desorption. The enrichment of low molecular weight proteins captured from human serum on mesoporous silica thin films fabricated by both methodologies were investigated by comparison of their MALDI-TOF MS profiles. This novel on-chip fractionation technology offers advantages in recovering the low molecular weight peptides from human serum, which has been recognized as an informative resource for early diagnosis of cancer and other diseases.
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影响因子：
4.4
作者：
Castagna, A;Cecconi, D;Rigetti, PG
通讯作者：
Rigetti, PG
影响因子：
4.4
作者：
Gaspari, M;Cheng, MMC;Ferrari, M
通讯作者：
Ferrari, M
DOI：
10.1016/j.jchromb.2006.09.038
发表时间：
2007-02-15
影响因子：
3
作者：
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影响因子：
3.5
作者：
Fountoulakis, M;Juranville, JF;Langen, H
通讯作者：
Langen, H
影响因子：
4.4
作者：
Jin, WH;Dai, J;Zeng, R
通讯作者：
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