Impact of disabled circadian clock on yellow fever mosquito Aedes aegypti fitness and behaviors.
Impact of disabled circadian clock on yellow fever mosquito Aedes aegypti fitness and behaviors.
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DOI：
10.1038/s41598-022-10825-5
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发表时间：
2022-04-27
影响因子：
4.6
通讯作者：
Slotman, Michel A.
中科院分区：
文献类型：
--
作者：
Shetty, Vinaya;Meyers, Jacob, I;Zhang, Ying;Merlin, Christine;Slotman, Michel A.
与其他昆虫一样，埃及伊蚊在寻找寄主和交配方面表现出强烈的日常模式。大多数这些行为被认为是在生物钟的控制下，生物钟是一种内源性计时机制，依赖于驱动节律性生理和行为的转录/翻译负反馈循环。检查生物钟和各种 Ae 之间的联系。针对埃及伊蚊的行为，我们使用 CRISPR/Cas9 敲除了核心时钟基因周期。 我们发现，包括 AeCyc−/− 在内的 7 个昼夜节律基因的 mRNA 表达节律模式和强度在昼夜周期以及持续黑暗条件下发生了变化。我们进一步表明，突变型 CYC 蛋白无法与 CLK 形成二聚体来刺激每个表达，并且内源时钟在 AeCyc−/− 蚊子中被禁用。 AeCyc−/− 不表现出野生型的双峰运动活动模式，对宿主气味的反应显着降低，卵孵化率降低，胚胎发育延迟，成虫存活率和交配成功率降低。然而令人惊讶的是，AeCyc−/− 雌性的吸血倾向明显高于野生型雌性。与最近关于蚊子生物学关键方面的生物钟控制的其他工作一起，我们关于周期 KO 如何影响蚊子行为和健康的数据为进一步研究连接蚊子内源时钟与其载体能力的途径提供了基础。
Like other insects, Aedes aegypti displays strong daily patterns in host seeking and mating. Much of these behaviors are believed to be under the control of a circadian clock, an endogenous timekeeping mechanism relying on transcriptional/translational negative feedback loops that drive rhythmic physiology and behavior. To examine the connection between the circadian clock and various Ae. aegypti behaviors, we knocked out the core clock gene cycle using CRISPR/Cas9. We found that the rhythmic pattern and intensity of mRNA expression of seven circadian genes, including AeCyc−/−, were altered across the day/night cycle as well as in constant darkness conditions. We further show that the mutant CYC protein is incapable of forming a dimer with CLK to stimulate per expression and that the endogenous clock is disabled in AeCyc−/− mosquitoes. AeCyc−/− do not display the bimodal locomotor activity pattern of wild type, have a significantly reduced response to host odor, reduced egg hatching rates, delayed embryonic development and reduced adult survival and mating success. Surprisingly however, the propensity to blood feed in AeCyc−/− females is significantly higher than in wildtype females. Together with other recent work on the circadian clock control of key aspects of mosquito biology, our data on how cycle KO affects mosquito behavior and fitness provides a basis for further work into the pathways that connect the mosquito endogenous clock to its vector competence.
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影响因子：
5.3
作者：
Granados-Fuentes, Daniel;Tseng, Alan;Herzog, Erik D.
通讯作者：
Herzog, Erik D.
影响因子：
3.8
作者：
KYRIACOU, CP;OLDROYD, M;HILL, M
通讯作者：
HILL, M
影响因子：
18.2
作者：
Allada R;Chung BY
通讯作者：
Chung BY
影响因子：
64.8
作者：
JONES, MDR;REITER, P
通讯作者：
REITER, P
DOI：
10.2987/8756-971x(2007)23
发表时间：
2007-01-01
影响因子：
1
作者：
Platzer, Edward G. -
通讯作者：
Platzer, Edward G. -