The mechanism for the radioprotective effects of zymosan-A in mice.
The mechanism for the radioprotective effects of zymosan-A in mice.
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酵母聚糖-A对小鼠辐射防护作用的机制
DOI：
10.1111/jcmm.13538
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发表时间：
2018-04
影响因子：
5.3
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Du J;Zhang P;Zhao H;Dong S;Yang Y;Cui J;Gao F;Cai J;Liu C
在我们之前的研究中证明了Zymosan-A通过Toll样受体2保护造血系统免受辐射诱导的损伤。在这项研究中，我们研究了Zymosan-A辐射防护作用的潜在机制。在电离辐射前24和2小时，通过腹膜注射用酵母聚糖-A（50 mg/kg，溶于NS）处理小鼠。通过流式细胞术测定骨髓细胞凋亡和IL-6、IL-12、G-CSF和GM-CSF水平。通过γ-H2 AX焦点测定来确定DNA损伤。此外，进行RNA测序以鉴定差异表达基因（DEG）。酵母聚糖A保护骨髓细胞免受辐射诱导的细胞凋亡，骨髓细胞中的IL-6、IL-12、G-CSF和GM-CSF上调。Zymosan-A还保护细胞免受辐射诱导的DNA损伤。此外，RNA测序分析显示，Zymosan-A诱导了131个参与免疫系统过程和炎症反应调节的DEG。DEG主要聚集在18条KEGG通路上，这些通路也与免疫系统过程相关。酵母聚糖A保护骨髓细胞免受辐射诱导的细胞凋亡，并上调IL-6、IL-12、G-CSF和GM-CSF。此外，Zymosan-A还可能通过调节免疫系统过程和炎症反应表现出辐射防护作用。这些结果为Zymosan-A的辐射防护作用提供了新的知识。
It proved that Zymosan‐A protected the haematopoietic system from radiation‐induced damage via Toll‐Like Receptor2 in our previous study. In this study, we investigated the potential mechanism for the radioprotective effects of Zymosan‐A. The mice were treated with Zymosan‐A (50 mg/kg, dissolved in NS) via peritoneal injection 24 and 2 hours before ionizing radiation. Apoptosis of bone marrow cells and the levels of IL‐6, IL‐12, G‐CSF and GM‐CSF were evaluated by flow cytometry assay. DNA damage was determined by γ‐H2AX foci assay. In addition, RNA sequencing was performed to identify differentially expressed genes (DEGs). Zymosan‐A protected bone marrow cells from radiation‐induced apoptosis, up‐regulated IL‐6, IL‐12, G‐CSF and GM‐CSF in bone marrow cells. Zymosan‐A also protected cells from radiation‐induced DNA damage. Moreover, RNA sequencing analysis revealed that Zymosan‐A induced 131 DEGs involved in the regulation of immune system process and inflammatory response. The DEGs were mainly clustered in 18 KEGG pathways which were also associated with immune system processes. Zymosan‐A protected bone marrow cells from radiation‐induced apoptosis and up‐regulated IL‐6, IL‐12, G‐CSF and GM‐CSF. Moreover, Zymosan‐A might also exhibit radioprotective effects through regulating immune system process and inflammatory response. These results provided new knowledge regarding the radioprotective effect of Zymosan‐A.
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DOI：
10.1126/science.aaf7532
发表时间：
2016-11-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hu B;Jin C;Li HB;Tong J;Ouyang X;Cetinbas NM;Zhu S;Strowig T;Lam FC;Zhao C;Henao-Mejia J;Yilmaz O;Fitzgerald KA;Eisenbarth SC;Elinav E;Flavell RA
通讯作者：
Flavell RA
影响因子：
6
作者：
Lee YK;Kang M;Choi EY
通讯作者：
Choi EY
影响因子：
3.1
作者：
Stansborough, Romany L.;Bateman, Emma H.;Gibson, Rachel J.
通讯作者：
Gibson, Rachel J.
影响因子：
9
作者：
通讯作者：
--
影响因子：
5.2
作者：
Mavragani IV;Nikitaki Z;Souli MP;Aziz A;Nowsheen S;Aziz K;Rogakou E;Georgakilas AG
通讯作者：
Georgakilas AG