Transcriptome Analysis Reveals a Potential Role of Benzoxazinoid in Regulating Stem Elongation in the Wheat Mutant qd.
Transcriptome Analysis Reveals a Potential Role of Benzoxazinoid in Regulating Stem Elongation in the Wheat Mutant qd.
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转录组分析揭示苯并恶嗪类化合物在调节小麦突变体 qd 茎伸长中的潜在作用
DOI：
10.3389/fgene.2021.623861
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发表时间：
2021
影响因子：
3.7
通讯作者：
Liu L
中科院分区：
文献类型：
--
作者：
Xu D;Xie Y;Guo H;Zeng W;Xiong H;Zhao L;Gu J;Zhao S;Ding Y;Liu L
谷类作物的茎既为抗倒伏提供机械支持，又为生殖器官提供养分。伸长是冬小麦(Triticum aestivum L.)产量决定的关键阶段，从第一个可察觉的节位开始到开花。此前，我们研究了重离子束引发的小麦突变体Qd，该突变体在不影响成熟株高的情况下表现出茎伸长模式的改变。在本研究中，我们利用转录组数据进一步分析了突变体的茎发育特征。在该项目中，每个生物样本获得了超过40.87Mb的清洁读数，其中包括至少36.61Mb的唯一定位读数。我们利用我们的转录组数据识别了124,971个基因。在这些基因中，有4340个差异表达基因(DEG)在QD植物和野生型(WT)植物之间被鉴定。与WT植物相比，QD植物表达了2,462个DEG，表达水平下调，1878个DEG表达上调。利用DEXSeq，我们鉴定了2391个计数框，对应于1148个基因，其中289个在DEG分析中也被发现，显示了QD和WT之间的差异。利用QD和WT的5,199个差异表达基因进行GO和KEGG分析。GO分析显著丰富了蛋白质-DNA复合体亚基组织、蛋白质-DNA复合体组装、核小体组织、核小体组装和染色质组装等生物学过程。然而，KEGG分析仅丰富了与苯并恶嗪生物合成途径相关的基因。编码苯并恶嗪生物合成酶Bx1、Bx3、Bx4、Bx5和Bx8_9的基因被证实在QD和WT之间存在差异表达。我们的结果表明，苯并恶嗪类化合物可能在调节QD的茎伸长表型中起关键作用。
The stems of cereal crops provide both mechanical support for lodging resistance and a nutrient supply for reproductive organs. Elongation, which is considered a critical phase for yield determination in winter wheat (Triticum aestivum L.), begins from the first node detectable to anthesis. Previously, we characterized a heavy ion beam triggered wheat mutant qd, which exhibited an altered stem elongation pattern without affecting mature plant height. In this study, we further analyzed mutant stem developmental characteristics by using transcriptome data. More than 40.87 Mb of clean reads including at least 36.61 Mb of unique mapped reads were obtained for each biological sample in this project. We utilized our transcriptome data to identify 124,971 genes. Among these genes, 4,340 differentially expressed genes (DEG) were identified between the qd and wild-type (WT) plants. Compared to their WT counterparts, qd plants expressed 2,462 DEGs with downregulated expression levels and 1878 DEGs with upregulated expression levels. Using DEXSeq, we identified 2,391 counting bins corresponding to 1,148 genes, and 289 of them were also found in the DEG analysis, demonstrating differences between qd and WT. The 5,199 differentially expressed genes between qd and WT were employed for GO and KEGG analyses. Biological processes, including protein-DNA complex subunit organization, protein-DNA complex assembly, nucleosome organization, nucleosome assembly, and chromatin assembly, were significantly enriched by GO analysis. However, only benzoxazinoid biosynthesis pathway-associated genes were enriched by KEGG analysis. Genes encoding the benzoxazinoid biosynthesis enzymes Bx1, Bx3, Bx4, Bx5, and Bx8_9 were confirmed to be differentially expressed between qd and WT. Our results suggest that benzoxazinoids could play critical roles in regulating the stem elongation phenotype of qd.
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影响因子：
6.9
作者：
Borràs-Gelonch G;Rebetzke GJ;Richards RA;Romagosa I
通讯作者：
Romagosa I
影响因子：
5.6
作者：
Guo Z;Chen D;Schnurbusch T
通讯作者：
Schnurbusch T
DOI：
10.1071/pp00021
发表时间：
2000-01-01
期刊：
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY
影响因子：
--
作者：
Miralles, DJ;Richards, RA;Slafer, GA
通讯作者：
Slafer, GA
影响因子：
11.4
作者：
Jiang, Jia-Fu;Xu, Yun-Yuan;Chong, Kang
通讯作者：
Chong, Kang
影响因子：
18
作者：
Gomez-Ariza, Jorge;Brambilla, Vittoria;Fornara, Fabio
通讯作者：
Fornara, Fabio