Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device.
Fast detection of SARS-CoV-2 RNA via the integration of plasmonic thermocycling and fluorescence detection in a portable device.
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DOI：
10.1038/s41551-020-00654-0
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发表时间：
2020-12
影响因子：
28.1
通讯作者：
Cheon J
中科院分区：
文献类型：
--
作者：
Cheong J;Yu H;Lee CY;Lee JU;Choi HJ;Lee JH;Lee H;Cheon J
目前COVID-19确认的金标准是RT-PCR，它具有高灵敏度和特异性，但周转时间长是一个缺点。在这里，我们介绍了一个nanoPCR平台，它可以在RT-PCR速度上取得突破，并分散诊断测试。一个关键的创新是利用磁等离子体纳米粒子的双重目的：通过等离子体加热和增强信号检测通过磁荧光开关的快速热循环。我们进一步将这些功能集成到便携式系统中，以简化护理点（POC）COVID-19诊断，从采样到结果的“一步完成”方法。当应用于临床COVID-19样本时，nanoPCR实现了与台式仪器相当的灵敏度和特异性分析性能，但在18分钟内完成了整个检测（而常规RT-PCR为2.5小时），这使其成为快速POC诊断的理想选择。该平台技术易于扩展，可实现更高的通量，并可扩展到其他疾病靶标，有望在现场分子检测中产生更广泛的影响。
The current gold standard for COVID-19 confirmation is RT-PCR, which possesses high sensitivity and specificity, but a long turnaround time has been a drawback. Here, we introduce a nanoPCR platform that can yield breakthroughs in RT-PCR speed and decentralize the diagnostic testing. A key innovation is to exploit magneto-plasmonic nanoparticles for dual purposes: fast thermocycling through plasmonic heating and enhanced signal detection via magnetic fluorescence switch. We further integrated these functionalities into a portable system to streamline point-of-care (POC) COVID-19 diagnostics with a “one-step-finish” approach from sampling to result. When applied with clinical COVID-19 samples, nanoPCR achieved an analytical performance of sensitivity and specificity comparable to that of a benchtop instrument but completed the entire detection within 18 minutes (vs. 2.5 hours for regular RT-PCR), which makes it ideal for rapid POC diagnostics. This platform technology is readily scalable for higher throughput and expandable to other disease targets, promising broader impacts in on-site molecular testing.
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影响因子：
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Huggett JF