Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish.
Polychlorinated biphenyl (118) activates osteoclasts and induces bone resorption in goldfish.
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DOI：
10.1007/s11356-012-1347-5
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Suzuki N
中科院分区：
文献类型：
--
作者：
Yachiguchi K;Matsumoto N;Haga Y;Suzuki M;Matsumura C;Tsurukawa M;Okuno T;Nakano T;Kawabe K;Kitamura K;Toriba A;Hayakawa K;Chowdhury VS;Endo M;Chiba A;Sekiguchi T;Nakano M;Tabuchi Y;Kondo T;Wada S;Mishima H;Hattori A;Suzuki N
为了分析多氯联苯（PCB）118对鱼骨代谢的影响，我们检测了注射PCB（118）的金鱼鳞片中的成骨细胞和成骨细胞活性以及血浆钙水平。此外，在体外研究PCB（118）对破骨细胞和成骨细胞的影响。未成熟的金鱼，其中内源性类固醇的影响是可以忽略不计的，被使用。将PCB（118）以10 ppm的浓度溶解在二甲基亚砜中。在PCB（118）注射（100纳克/克体重）后第1天和第2天，测量骨细胞和成骨细胞的活动以及血浆钙水平。然后，在体外研究中，检查了成骨细胞和成骨细胞的活性以及每种标记物mRNA的表达。在第2天，PCB（118）注射金鱼的鳞片骨细胞活性显著增加，而成骨细胞活性没有显著变化。与骨细胞活性相对应，PCB（118）给药后2天血浆钙水平显著升高。体外实验中，标志酶活性和mRNA表达也发生了骨细胞活化。因此，我们得出结论，PCB（118）破坏骨代谢的金鱼在体内和体外实验。
To analyze the effect of polychlorinated biphenyl (PCB) 118 on fish bone metabolism, we examined osteoclastic and osteoblastic activities, as well as plasma calcium levels, in the scales of PCB (118)-injected goldfish. In addition, effect of PCB (118) on osteoclasts and osteoblasts was investigated in vitro. Immature goldfish, in which the endogenous effects of sex steroids are negligible, were used. PCB (118) was solubilized in dimethyl sulfoxide at a concentration of 10 ppm. At 1 and 2 days after PCB (118) injection (100 ng/g body weight), both osteoclastic and osteoblastic activities, and plasma calcium levels were measured. In an in vitro study, then, both osteoclastic and osteoblastic activities as well as each marker mRNA expression were examined. At 2 days, scale osteoclastic activity in PCB (118)-injected goldfish increased significantly, while osteoblastic activity did not change significantly. Corresponding to osteoclastic activity, plasma calcium levels increased significantly at 2 days after PCB (118) administration. Osteoclastic activation also occurred in the marker enzyme activities and mRNA expressions in vitro. Thus, we conclude that PCB (118) disrupts bone metabolism in goldfish both in vivo and in vitro experiments.
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影响因子：
4.5
作者：
Lind, PM;Eriksen, EF;Sahlin, L
通讯作者：
Sahlin, L
DOI：
10.2215/cjn.05910809
发表时间：
2010-01-01
影响因子：
9.8
作者：
Peacock, Munro
通讯作者：
Peacock, Munro
影响因子：
4.5
作者：
Olufsen, Marianne;Arukwe, Augustine
通讯作者：
Arukwe, Augustine
影响因子：
11.4
作者：
Hope, Bruce K.
通讯作者：
Hope, Bruce K.
DOI：
10.1016/0300-9629(77)90455-8
发表时间：
1977-01-01
影响因子：
2.3
作者：
MUGIYA, Y;WATABE, N
通讯作者：
WATABE, N