Giant extracellular matrix binding protein expression in Staphylococcus epidermidis is regulated by biofilm formation and osmotic pressure.
Giant extracellular matrix binding protein expression in Staphylococcus epidermidis is regulated by biofilm formation and osmotic pressure.
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DOI：
10.1007/s00284-013-0316-7
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发表时间：
2013-06
影响因子：
2.6
通讯作者：
Bryers, James D.
中科院分区：
文献类型：
--
作者：
Linnes, Jacqueline C.;Ma, Hongyan;Bryers, James D.
表皮葡萄球菌是一种机会性细菌，作为一种皮肤微生物和血管病原体而蓬勃发展。色葡萄据报道，表皮细胞外基质结合蛋白（Embp）是一种毒力因子，参与医疗器械植入物的定殖和随后的生物膜形成。在这里，我们表征的表达模式Embp在渗透压和生物膜文化，以及在高渗透压应力下，代表的皮肤的皮肤环境。无渗透压的Embp表达与渗透压培养和贴壁培养相似。此外，通过NaCl的渗透胁迫引起embp的表达略有增加，在反渗透培养。然而，在贴壁培养中，与对照组相比，NaCl使embp表达增加了100倍，并与生物膜形态改变相吻合。结果表明，Embp的核心作用在于在皮肤定植，稳定细胞壁对渗透压应力，而不是作为一个毒力因子促进粘附。
Staphylococcus epidermidis is an opportunistic bacterium that thrives as a commensal cutaneous organism and as a vascular pathogen. The S. epidermidis extracellular matrix binding protein (Embp) has been reported to be a virulence factor involved in colonization of medical device implants and subsequent biofilm formation. Here, we characterize the expression patterns of Embp in planktonic and biofilm cultures, as well as under high osmotic stresses that typify the commensal environment of the skin. Embp expression without osmotic stress was similar for planktonic and adherent cultures. Addition of osmotic stress via NaCl caused slight increases in embp expression in planktonic cultures. However, in adherent cultures a 100-fold increase in embp expression with NaCl versus controls occurred and coincided with altered biofilm morphology. Results suggest that the central role of Embp lies in commensal skin colonization, stabilizing the cell wall against osmotic stresses, rather than as a virulence factor promoting adhesion.
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DOI：
10.1128/cdli.12.1.93-100.2005
发表时间：
2005-01-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Sun, DQ;Accavitti, MA;Bryers, JD
通讯作者：
Bryers, JD
影响因子：
3.1
作者：
Xu, L;Li, HL;Gao, Q
通讯作者：
Gao, Q
影响因子：
3.1
作者：
MACK, D;SIEMSSEN, N;LAUFS, R
通讯作者：
LAUFS, R
影响因子：
3.2
作者：
VIJARANAKUL, U;NADAKAVUKAREN, MJ;JAYASWAL, RK
通讯作者：
JAYASWAL, RK
影响因子：
6.4
作者：
Yao, YF;Sturdevant, DE;Otto, M
通讯作者：
Otto, M