NS-Pten knockout mice show sex- and age-specific differences in ultrasonic vocalizations.
NS-Pten knockout mice show sex- and age-specific differences in ultrasonic vocalizations.
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DOI：
10.1002/brb3.857
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发表时间：
2017-11
影响因子：
3.1
通讯作者：
Lugo JN
中科院分区：
文献类型：
--
作者：
Binder MS;Lugo JN
本研究的目的是确定与野生型雄性和雌性小鼠相比，神经元亚群特异性（NS-Pten）敲除雄性和雌性小鼠中新生儿超声发声USV的定量和定性变化。在自闭症样表型的发展中起关键作用的一个信号级联是PI 3 K/Akt/mTOR通路。说明这种联系的小鼠模型包括Fmr 1，Tsc 1和NS-Pten-缺陷小鼠。虽然许多研究已经研究了Fmr 1敲除和Tsc 1异源小鼠的超声发声，但没有研究使用全光谱记录系统在NS-Pten敲除小鼠中研究USV。我们在出生后第8天和第11天记录了NS-Pten野生型和基因敲除雄性和雌性小鼠的超声发声。在这些日子里，我们测量了幼崽从母亲身边离开时发出的叫声的数量和质量。我们发现，敲除基因的幼崽发出的声音更少（p <0.05）。敲除雄性在第8天具有较短的持续时间和较低的峰值振幅的叫声，而在第11天显示出较短的持续时间、较低的峰值振幅以及较高的峰值和基频（p < .001）。敲除雌性在第8天以较低的峰值振幅和基频发声，并且峰值频率较高，而在第11天显示出较短的持续时间和较高的峰值和基频（p <0.001）。光谱分析还揭示了基因型和性别的呼叫类型的显着差异（p <0.05）。这些发现表明，NS-Pten的缺失导致两种性别的发声显著减少。此外，我们的研究结果表明，在其他mTOR模型中观察到的异常发声和呼叫持续时间增加也存在于NS-Pten敲除小鼠中。我们的研究提供了mTOR信号过度活跃和新生儿超声发声之间联系的证据。
The goal of this study was to identify changes in quantitative and qualitative aspects of neonatal ultrasonic vocalizations USVs in neuron‐subset specific (NS‐Pten) knockout males and females when compared with wild‐type male and female mice. One signaling cascade that plays a crucial role in the development of an autistic‐like phenotype is the PI3K/Akt/mTOR pathway. Mouse models that illustrate this connection include Fmr1, Tsc1, and NS‐Pten‐deficient mice. While numerous studies have investigated ultrasonic vocalizations in Fmr1 knockout and Tsc1 heterogenous mice, none have investigated USVs in NS‐Pten knockout mice using a full spectrum recording system. We recorded ultrasonic vocalizations from NS‐Pten wild‐type and knockout male and female mice on postnatal days 8 and 11. On these days, we measured the number and quality of calls emitted from pups when they were removed from their mothers. We found that knockout pups emitted fewer vocalizations for both sexes (p < .05). Knockout males had calls of a shorter duration and lower peak amplitude on day 8, while showing a shorter duration, lower peak amplitude, and higher peak and fundamental frequency on day 11 (p < .001). Knockout females vocalized at a lower peak amplitude and fundamental frequency, and a higher peak frequency on day 8, while showing a shorter duration and a higher peak and fundamental frequency on day 11 (p < .001). Spectrographic analyses also revealed significant differences in call type for both genotypes and sexes (p < .05). These findings demonstrate that deletion of NS‐Pten results in significant decreases in vocalizations across both sexes. Additionally, our findings indicate that the aberrant vocalizations and increased call duration seen in other mTOR models are also present in NS‐Pten knockout mice. Our study provides evidence of a connection between hyperactive mTOR signaling and neonatal ultrasonic vocalizations.
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影响因子：
4.7
作者：
Nagarajan, Raman P.;Patzel, Katherine A.;LaSalle, Janine M.
通讯作者：
LaSalle, Janine M.
DOI：
10.1037/h0077287
发表时间：
1976-01-01
期刊：
JOURNAL OF COMPARATIVE AND PHYSIOLOGICAL PSYCHOLOGY
影响因子：
--
作者：
SMITH, JC
通讯作者：
SMITH, JC
影响因子：
25
作者：
Belmonte, Matthew K.;Bourgeron, Thomas
通讯作者：
Bourgeron, Thomas
影响因子：
2.6
作者：
Rapin, Isabelle;Tuchman, Roberto F.
通讯作者：
Tuchman, Roberto F.
影响因子：
3.7
作者：
Scattoni, Maria Luisa;Gandhy, Shruti U.;Ricceri, Laura;Crawley, Jacqueline N.
通讯作者：
Crawley, Jacqueline N.