Kapok Fiber: A Natural Biomaterial for Highly Specific and Efficient Enrichment of Sialoglycopeptides.

Kapok Fiber: A Natural Biomaterial for Highly Specific and Efficient Enrichment of Sialoglycopeptides.
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木棉纤维:一种用于高度特异性和高效富集唾液酸糖肽的天然生物材料。

DOI:
10.1021/acs.analchem.5b04014
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发表时间:
2016-01
影响因子:
7.4
通讯作者:
Li Zhili
Li Zhili
中科院分区:
化学1区
文献类型:
--
作者:
Liu Yujie;Liu Yuanjun;Zhang Dan;Zhang Ruiqing;Li Zhili

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与正常细胞相比,癌症发展和慢性疾病与终止于癌细胞表面蛋白和脂质的唾液酸聚糖的过度表达有关。从复杂肽混合物中分离和检测唾液酸糖肽仍然是一个挑战,因为它们的丰度低,电离度低,并且在分析过程中会损失唾液酸残基和水分子。在这项研究中,木棉纤维,一种来自木棉树(木棉)的天然纤维,已经显示出特异性和有效地富集唾液酸糖肽的优异能力,而不损失唾液酸残基和来自唾液酸聚糖的水分子。木棉纤维表面的主要成分是异辛基木质素和愈创木基木质素,它们在从复杂的肽混合物中分离唾液酸糖肽中起重要作用。
Cancer development and chronic diseases are associated with the overexpression of sialoglycans terminated to the surface proteins and lipids of cancer cells compared with normal cells. The isolation and detection of sialoglycopeptides from complex peptides mixture still remain challenges due to their low abundance, low ionization, and losses of sialic acid residues and water molecule during analytical processes. In this study, kapok fiber, a natural fiber derived from the kapok tree (Bombax ceiba L.), has shown excellent capability to specifically and efficiently enrich sialoglycopeptides, without losses of sialic acid residues and water molecule from sialoglycans. The main components on the surface of kapok fiber are syringyl and guaiacyl lignins which play an important role in isolating sialoglycopeptides from complex peptide mixtures.
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