Semen-Derived Exosomes Mediate Immune Escape and Transmission of Reticuloendotheliosis Virus.
Semen-Derived Exosomes Mediate Immune Escape and Transmission of Reticuloendotheliosis Virus.
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精液衍生的外泌体介导了网状内膜病毒的免疫逃逸和传播。
DOI：
10.3389/fimmu.2021.735280
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发表时间：
2021
影响因子：
7.3
通讯作者：
Zhao P
中科院分区：
文献类型：
--
作者：
Su Q;Zhang Y;Cui Z;Chang S;Zhao P
网状内皮组织增生症病毒(REV)引起家禽免疫抑制病，给世界范围内造成巨大的经济负担。最近的证据表明，REV可以进入精液，然后诱导人工授精，但病毒如何进入精液鲜为人知。越来越多的研究表明，外切体是病毒传播的载体，但外切体在通过精液传播病毒方面的作用尚不清楚。在本研究中，从REV阳性的精液中提纯的外切体经逆转录-聚合酶链式反应和质谱分析证明含有病毒基因组RNA和病毒蛋白，这也可以在体内和体外建立有效的感染和逃避REV特异性的中和抗体。更重要的是，与游离病毒粒子引起的感染相比，外切体更有效地确保了病毒的有效感染和复制，这也可以帮助REV妥协宿主免疫反应的功效。综上所述，本研究证明精液来源的外切体可以抑制REV的传播和免疫逃逸，这意味着REV进入精液并导致垂直传播的一种新机制。
Reticuloendotheliosis virus (REV) causes immune-suppression disease in poultry, leading to a significant economic burden worldwide. Recent evidence demonstrated that the REV can enter the semen and then induce artificial insemination, but how the virus gets into semen was little known. Accumulating studies indicated that exosomes serve as vehicles for virus transmission, but the role of exosomes in viral shedding through the semen remains unclear. In this study, exosomes purified from the REV-positive semen were shown with reverse transcription-PCR and mass spectrometry to contain viral genomic RNA and viral proteins, which could also establish productive infections both in vivo and in vitro and escape from the REV-specific neutralizing antibodies. More importantly, compared with the infection caused by free virions, the exosome is more efficient for the virus to ensure effective infection and replication, which can also help the REV compromise the efficacy of the host immune response. In summary, this study demonstrated that semen-derived exosomes can medicate the transmission and immune escape of REV, implicating a novel mechanism for REV entering the semen and leading to vertical transmission.
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影响因子：
64.8
作者：
Feng Z;Hensley L;McKnight KL;Hu F;Madden V;Ping L;Jeong SH;Walker C;Lanford RE;Lemon SM
通讯作者：
Lemon SM
影响因子：
7.3
作者：
Lundy SK;Klinker MW;Fox DA
通讯作者：
Fox DA
影响因子：
3.3
作者：
Jiang, Lili;Qi, Xiaole;Wang, Xiaomei
通讯作者：
Wang, Xiaomei
影响因子：
24
作者：
Marbán E
通讯作者：
Marbán E
DOI：
10.1046/j.1439-0450.2001.00472.x
发表时间：
2001-09-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Hafez, HM
通讯作者：
Hafez, HM