Contribution of the cytoskeleton to mechanosensitivity reported by dinoflagellate bioluminescence
Contribution of the cytoskeleton to mechanosensitivity reported by dinoflagellate bioluminescence
复制标题
甲藻生物发光报告的细胞骨架对机械敏感性的贡献
作者：
J. Stires;M. Latz
细胞骨架对于细胞力学和感知细胞外物理环境是至关重要的。这项研究的目的是利用其固有的生物发光作为机械转导的快速报告，来研究皮层细胞骨架在单细胞原生动物--海洋多年生甲藻--机械敏感性中的作用。药物治疗解决了由于细胞骨架立即中断而产生的影响，而不是由于细胞骨架在从明到暗的相变期间重塑造成的影响。通过免疫组织化学标记的微管和鬼臼蛋白标记的F-肌动蛋白的激光共聚焦扫描显微镜观察细胞骨架，并根据在黑暗阶段测量的对机械刺激的生物发光反应来评估机械敏感性。在从亮到暗的过渡阶段后，Latrunculin B处理导致皮质F-肌动蛋白的一些破坏，对皮质微管没有观察到的影响，并部分抑制了生物发光反应。米沙林可使微管去极化，完全破坏微管网络和皮质F-肌动蛋白，并部分抑制生物发光。这些结果表明，尽管细胞骨架被破坏，但细胞仍保持一定的机械敏感性；将机械敏感性与完整的F-肌动蛋白联系起来；F-肌动蛋白与组成皮质细胞骨架的微管之间存在密切联系；证实肌动蛋白细胞骨架对闪烁体的移位有很强的贡献，即含有发光化学物质的囊泡；并支持肌动蛋白细胞骨架在闪烁体与液泡膜的结合中的作用。
The cytoskeleton is crucial to cell mechanics and sensing the extracellular physical environment. The objective of this study was to examine the role of the cortical cytoskeleton in mechanosensitivity in a unicellular protist, the marine dinoflagellate Lingulodinium polyedra, using its intrinsic bioluminescence as a rapid reporter of mechanotransduction. Pharmacological treatments resolved effects due to immediate cytoskeleton disruption from those due to cytoskeletal remodeling during the light to dark phase transition. The cytoskeleton was visualized by confocal laser scanning microscopy of immunohistochemically labeled microtubules and phalloidin labeled F‐actin, and mechanosensitivity assessed based on the bioluminescence response to mechanical stimulation measured during the dark phase. Latrunculin B treatment after the transition from the light to dark phase resulted in some disruption of cortical F‐actin, no observed effect on the cortical microtubules, and partial inhibition of the bioluminescence response. Treatment with oryzalin, which depolarizes microtubules, completely disrupted the microtubule network and cortical F‐actin, and partially inhibited bioluminescence. These results demonstrate that cells retain some mechanosensitivity despite a disrupted cytoskeleton; link mechanosensitivity to intact F‐actin; show a close connection between F‐actin and microtubules comprising the cortical cytoskeleton; confirm a strong contribution of the actin cytoskeleton to the translocation of scintillons, vesicles containing the luminescent chemistry; and support the role of the actin cytoskeleton in the association of scintillons with the vacuole membrane.
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影响因子：
19
作者：
Diz-Muñoz A;Fletcher DA;Weiner OD
通讯作者：
Weiner OD
DOI：
10.1073/pnas.95.5.2515
发表时间：
1998-03-03
影响因子：
11.1
作者：
Gudi, S;Nolan, JP;Frangos, JA
通讯作者：
Frangos, JA
DOI：
10.1152/ajpheart.2000.278.4.h1401
发表时间：
2000-04-01
影响因子：
4.8
作者：
Haidekker, MA;L'Heureux, N;Frangos, JA
通讯作者：
Frangos, JA
影响因子：
2.5
作者：
Ketene, Alperen N.;Roberts, Paul C.;Agah, Masoud
通讯作者：
Agah, Masoud
影响因子：
7.5
作者：
Waterman-Storer, CM;Salmon, ED
通讯作者：
Salmon, ED