Replication of biocompatible, nanotopographic surfaces.
Replication of biocompatible, nanotopographic surfaces.
复制标题
复制生物相容性纳米表面。
DOI：
10.1038/s41598-017-19008-z
复制
发表时间：
2018-01-12
影响因子：
4.6
通讯作者：
Fourkas JT
中科院分区：
文献类型：
--
作者：
Sun X;Hourwitz MJ;Baker EM;Schmidt BUS;Losert W;Fourkas JT
细胞感知和响应纳米形貌的能力被认为是许多生理过程（如细胞分化、免疫应答和伤口愈合）以及病理学（如癌症转移）的关键因素。为了了解纳米形貌如何影响细胞行为，需要新的技术来大规模生产生物相容的刚性纳米形貌表面。在这里，我们介绍了一种方法，用于快速和可再现的生产生物相容性，刚性，丙烯酸纳米地形表面，并与细胞实验的粘附促进分子的表面的功能化。复制品表面表现出高的光学透明度，这对于高分辨率的活细胞成像是有利的。作为一个有代表性的应用，我们证明了上皮细胞在由纳米级脊和凹槽组成的表面上形成局灶性粘连，并且局灶性粘连更喜欢定位在纳米脊上。我们进一步证明了F-肌动蛋白和微管都沿着沿着纳米脊排列，但只有F-肌动蛋白沿着沿着纳米槽排列。纳米地形表面的大规模生产打开了大门的物理线索的空间分布和动态的细胞内蛋白质的影响的调查，并在如细胞迁移，吞噬，分裂和分化的过程中的机械传感的机制的研究。
The ability of cells to sense and respond to nanotopography is being implicated as a key element in many physiological processes such as cell differentiation, immune response, and wound healing, as well as in pathologies such as cancer metastasis. To understand how nanotopography affects cellular behaviors, new techniques are required for the mass production of biocompatible, rigid nanotopographic surfaces. Here we introduce a method for the rapid and reproducible production of biocompatible, rigid, acrylic nanotopographic surfaces, and for the functionalization of the surfaces with adhesion-promoting molecules for cell experiments. The replica surfaces exhibit high optical transparency, which is advantageous for high-resolution, live-cell imaging. As a representative application, we demonstrate that epithelial cells form focal adhesions on surfaces composed of nanoscale ridges and grooves, and that the focal adhesions prefer to localize on the nanoridges. We further demonstrate that both F-actin and microtubules align along the nanoridges, but only F-actin aligns along the nanogrooves. The mass production of nanotopographic surfaces opens the door to the investigation of the effect of physical cues on the spatial distribution and the dynamics of intracellular proteins, and to the study of the mechanism of mechanosensing in processes such as cell migration, phagocytosis, division, and differentiation.
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影响因子：
17.1
作者：
Driscoll, Meghan K.;Sun, Xiaoyu;Guven, Can;Fourkas, John T.;Losert, Wolfgang
通讯作者：
Losert, Wolfgang
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1073/pnas.0903269107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Kilian, Kristopher A.;Bugarija, Branimir;Mrksich, Milan
通讯作者：
Mrksich, Milan
影响因子：
3.5
作者：
Kang, H;Lee, J;Lee, HH
通讯作者：
Lee, HH
影响因子：
3.7
作者：
McCann, Colin P.;Rericha, Erin C.;Parent, Carole A.
通讯作者：
Parent, Carole A.