Size selective sampling using mobile, 3D nanoporous membranes.
Size selective sampling using mobile, 3D nanoporous membranes.
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DOI：
10.1007/s00216-008-2538-2
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发表时间：
2009-02
影响因子：
4.3
通讯作者：
Gracias, David H.
中科院分区：
文献类型：
--
作者：
Randall, Christina L.;Gillespie, Aubri;Singh, Siddarth;Leong, Timothy G.;Gracias, David H.
关键词：
我们描述了三维（3D）膜的制造与精确图案化的表面纳米孔隙率和它们的利用率在尺寸选择性采样。该膜自组装为从光刻制造的2D模板的面尺寸为200 μm，体积为8 nL，和单分散孔的大小范围从约10 μm到100 nm的多孔立方体。与传统的采样和过滤方案相反，在传统的采样和过滤方案中，流体移动穿过静态膜，我们通过移动3D纳米多孔膜来演示采样。这种新方案允许在小体积中直接采样，几乎没有损失。将具有五个多孔面和一个开放面的膜移动通过流体以基于孔径取样并保留纳米级珠和细胞。此外，随后使用标准细胞培养方案培养保留在膜内的细胞，并在回收时进行增殖。
We describe the fabrication of three-dimensional (3D) membranes with precisely patterned surface nanoporosity and their utilization in size selective sampling. The membranes were self-assembled as porous cubes from lithographically fabricated 2D templates with face dimensions of 200 μm, volumes of eight nL, and monodisperse pores ranging in size from approximately 10 μm to 100 nm. As opposed to conventional sampling and filtration schemes where fluid is moved across a static membrane, we demonstrate sampling by instead moving the 3D nanoporous membrane through the fluid. This new scheme allows for straightforward sampling in small volumes, with little to no loss. Membranes with five porous faces and one open face were moved through fluids to sample and retain nanoscale beads and cells based on pore size. Additionally, cells retained within the membranes were subsequently cultured and multiplied using standard cell culture protocols upon retrieval.
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