Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance.
Digitally Driven Aerosol Jet Printing to Enable Customisable Neuronal Guidance.
复制标题
数字驱动的气溶胶喷气打印以实现可定制的神经元指导。
DOI：
10.3389/fcell.2021.722294
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
Harris RA
中科院分区：
文献类型：
--
作者：
Capel AJ;Smith MAA;Taccola S;Pardo-Figuerez M;Rimington RP;Lewis MP;Christie SDR;Kay RW;Harris RA
数字驱动的制造技术，如气溶胶喷射打印（AJP），可以为组织工程疾病建模和药物筛选领域的新能力做出重大贡献。AJP是一种新兴的非接触和无掩模印刷工艺，与其他图案化技术相比具有明显的优势，因为它提供了多种材料在平面和非平面表面上的通用、高分辨率、直写沉积。这项研究证明了AJP能够打印影响神经元引导的数字控制模式。这些由玻璃和聚（甲基丙烯酸3-磺丙钾）（PKSPMA）涂覆的玻璃表面上的图案化聚（3，4-亚乙基二氧噻吩）-聚（苯乙烯磺酸盐）（PEDOT：PSS）轨道组成，促进SH-SY 5 Y神经母细胞瘤细胞的选择性粘附。细胞吸引图案的最大高度≥0.2 μm，宽度和半高≥15 μm，Ra = 3.5 nm，RMS = 4.1。所开发的生物相容性PEDOT：PSS墨水显示出促进SH-SY 5 Y神经元细胞的粘附、生长和分化。SH-SY 5 Y细胞直接培养到这些功能表现出增加的细胞核和神经元排列在两个基板上。此外，当培养到PKSPMA表面上时，细胞对基底的粘附是选择性的，从而导致高度组织化的神经模式。这证明了通过计算机控制的过程快速和灵活地实现复杂和精确的细胞图案的能力。
Digitally driven manufacturing technologies such as aerosol jet printing (AJP) can make a significant contribution to enabling new capabilities in the field of tissue engineering disease modeling and drug screening. AJP is an emerging non-contact and mask-less printing process which has distinct advantages over other patterning technologies as it offers versatile, high-resolution, direct-write deposition of a variety of materials on planar and non-planar surfaces. This research demonstrates the ability of AJP to print digitally controlled patterns that influence neuronal guidance. These consist of patterned poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS) tracks on both glass and poly(potassium 3-sulfopropyl methacrylate) (PKSPMA) coated glass surfaces, promoting selective adhesion of SH-SY5Y neuroblastoma cells. The cell attractive patterns had a maximum height ≥0.2 μm, width and half height ≥15 μm, Ra = 3.5 nm, and RMS = 4.1. The developed biocompatible PEDOT:PSS ink was shown to promote adhesion, growth and differentiation of SH-SY5Y neuronal cells. SH-SY5Y cells cultured directly onto these features exhibited increased nuclei and neuronal alignment on both substrates. In addition, the cell adhesion to the substrate was selective when cultured onto the PKSPMA surfaces resulting in a highly organized neural pattern. This demonstrated the ability to rapidly and flexibly realize intricate and accurate cell patterns by a computer controlled process.
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影响因子：
2.8
作者：
Filipponi L;Livingston P;Kašpar O;Tokárová V;Nicolau DV
通讯作者：
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通讯作者：
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5.7
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