The Receptor-Like Kinase ERECTA Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density.
The Receptor-Like Kinase ERECTA Confers Improved Water Use Efficiency and Drought Tolerance to Poplar via Modulating Stomatal Density.
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受体样激酶 ERECTA 通过调节气孔密度提高杨树的水分利用效率和耐旱性。
DOI：
10.3390/ijms22147245
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发表时间：
2021-07-06
影响因子：
5.6
通讯作者：
Xia X
中科院分区：
文献类型：
--
作者：
Li H;Yang Y;Wang H;Liu S;Jia F;Su Y;Li S;He F;Feng C;Niu M;Wang J;Liu C;Yin W;Xia X
杨树是北温带最重要的树种之一，但杨树人工林水分密集。我们报道了CaMV 35S启动子驱动的PdERECTA基因在三倍体白杨中的过表达，该基因属于LRR-RLKS家族成员，可以提高三倍体白杨的水分利用效率和抗旱性。PdERECTA定位于质膜。过量表达植株的气孔密度较低，气孔大小较大。高表达品系的远轴气孔密度降低24-34%，气孔尺寸增大12-14%。气孔密度降低对蒸腾作用有明显的抑制作用，比对照低18-35%，瞬时水分利用效率在不同条件下比对照高14-63%。这些表型变化导致了抗旱性的提高。PdERECTA高表达植株不仅在停止浇水后存活时间更长，而且在有限水分条件下表现更好，因为它们具有更好的物理和光合作用条件，生长速度更快，生物量积累更高。综上所述，我们的数据表明，PdERECTA可以改变气孔发育模式，降低气孔密度，从而限制水分消耗，增强杨树的抗旱性。这使得PdERECTA树有望成为建立更多节水高效种植园的候选者。
Poplar is one of the most important tree species in the north temperate zone, but poplar plantations are quite water intensive. We report here that CaMV 35S promoter-driven overexpression of the PdERECTA gene, which is a member of the LRR-RLKs family from Populus nigra × (Populus deltoides × Populus nigra), improves water use efficiency and enhances drought tolerance in triploid white poplar. PdERECTA localizes to the plasma membrane. Overexpression plants showed lower stomatal density and larger stomatal size. The abaxial stomatal density was 24–34% lower and the stomatal size was 12–14% larger in overexpression lines. Reduced stomatal density led to a sharp restriction of transpiration, which was about 18–35% lower than the control line, and instantaneous water use efficiency was around 14–63% higher in overexpression lines under different conditions. These phenotypic changes led to increased drought tolerance. PdERECTA overexpression plants not only survived longer after stopping watering but also performed better when supplied with limited water, as they had better physical and photosynthesis conditions, faster growth rate, and higher biomass accumulation. Taken together, our data suggest that PdERECTA can alter the development pattern of stomata to reduce stomatal density, which then restricts water consumption, conferring enhanced drought tolerance to poplar. This makes PdERECTA trees promising candidates for establishing more water use efficient plantations.
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影响因子：
5.6
作者：
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3.5
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影响因子：
44.1
作者：
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影响因子：
3.5
作者：
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通讯作者：
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影响因子：
9.4
作者：
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通讯作者：
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