A general method for detecting protease activity via gelation and its application to artificial clotting.

A general method for detecting protease activity via gelation and its application to artificial clotting.
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DOI:
10.1039/c2cc31537h
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发表时间:
2012-06-04
期刊:
Chemical communications (Cambridge, England)
影响因子:
--
通讯作者:
Soellner MB
Soellner MB
中科院分区:
其他
文献类型:
--
作者:
Bremmer SC;Chen J;McNeil AJ;Soellner MB

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A modular system for detecting protease activity via enzyme-triggered gel formation is described. Protease-specific recognition sequences are utilized to achieve enzyme specificity. Artificial blood clotting is demonstrated by activating endogenous thrombin to trigger gelation in fibrinogen-deficient blood plasma.
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