Characterisation of superficial corneocytes in skin areas of the face exposed to prolonged usage of respirators by healthcare professionals during COVID-19 pandemic.
Characterisation of superficial corneocytes in skin areas of the face exposed to prolonged usage of respirators by healthcare professionals during COVID-19 pandemic.
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DOI：
10.1016/j.jtv.2023.02.007
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发表时间：
2023-05
影响因子：
2.5
通讯作者：
Bader, Dan L.
中科院分区：
文献类型：
--
作者：
Evora, Ana S.;Abiakam, Nkemjika;Jayabal, Hemalatha;Worsley, Peter R.;Zhang, Zhibing;Johnson, Simon A.;Adams, Michael J.;Bader, Dan L.
关键词：
在新冠肺炎大流行期间，医护人员长时间使用呼吸防护设备，这与对底层皮肤的有害影响有关。本研究旨在评估长期和连续使用呼吸器后角质层(SC)主要细胞(角质细胞)的变化。17名在医院日常工作中每天佩戴呼吸器的卫生工作者被招募到纵向队列研究中。通过胶带剥离从阴性对照部位(呼吸器外部区域)和与呼吸器接触的面颊收集角膜细胞。取角膜细胞三次，检测角化包膜(CES)和桥粒芯糖蛋白-1(DSG1)含量，作为未成熟CES和角化桥粒(CDS)的间接测量。在相同的调查地点，将这些数据与生物物理测量(透皮失水、TEWL和SC水合作用)进行比较。被试之间存在很大程度的变异性，未成熟CES和DSG1水平的最大变异系数分别为43%和30%。虽然观察到长时间使用呼吸器对角膜细胞的特性没有影响，但面颊处的CDS水平高于阴性对照部位(P<0.05)。此外，在长时间使用呼吸器后，低水平的未成熟CES与较大的TEWL值相关(p<0.01)。还注意到，未成熟的CES和CDS的比例较小，与自我报告的皮肤不良反应的发生率降低有关(P<；0.001)。这是第一个研究使用呼吸器后长时间机械负荷情况下角膜细胞特性变化的研究。尽管没有随着时间的推移而记录差异，但与阴性对照部位相比，负荷组脸颊中CDS和未成熟CES的水平始终更高，并与更多自我报告的皮肤不良反应数量呈正相关。需要进一步的研究来评估角膜细胞特征在评估健康和受损皮肤部位中的作用。
During the COVID-19 pandemic healthcare workers (HCWs) have used respiratory protective equipment for prolonged periods, which has been associated with detrimental effects on the underlying skin. The present study aims to evaluate changes in the main cells (corneocytes) of the stratum corneum (SC) following prolonged and consecutive use of respirators. 17 HCWs who wore respirators daily during routine hospital practice were recruited to a longitudinal cohort study. Corneocytes were collected via tape stripping from a negative control site (area outside the respirator) and from the cheek which was in contact with the device. Corneocytes were sampled on three occasions and analysed for the level of positive-involucrin cornified envelopes (CEs) and the amount of desmoglein-1 (Dsg1), as indirect measurements of immature CEs and corneodesmosomes (CDs), respectively. These were compared to biophysical measurements (Transepidermal water loss, TEWL, and SC hydration) at the same investigation sites. A large degree of inter-subject variability was observed, with maximum coefficients of variation of 43% and 30% for the level of immature CEs and Dsg1, respectively. Although it was observed that there was not an effect of prolonged respirator usage on the properties of corneocytes, the level of CDs was greater at the cheek than the negative control site (p < 0.05). Furthermore, low levels of immature CEs correlated with greater TEWL values after prolonged respirator application (p < 0.01). It was also noted that a smaller proportion of immature CEs and CDs was associated with a reduced incidence of self-reported skin adverse reactions (p < 0.001). This is the first study that investigated changes in corneocyte properties in the context of prolonged mechanical loading following respirator application. Although differences were not recorded over time, the levels of CDs and immature CEs were consistently higher in the loaded cheek compared to the negative control site and were positively correlated with a greater number of self-reported skin adverse reactions. Further studies are required to evaluate the role of corneocyte characteristics in the evaluation of both healthy and damaged skin sites.
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影响因子：
10.3
作者：
COMAISH, S;BOTTOMS, E
通讯作者：
BOTTOMS, E
影响因子：
2.3
作者：
Guneri, D.;Voegeli, R.;Rawlings, A. V.
通讯作者：
Rawlings, A. V.
影响因子：
5.5
作者：
Hua, Wei;Zuo, Ying;Li, Li
通讯作者：
Li, Li
DOI：
10.1016/j.jmbbm.2019.103391
发表时间：
2019-12-01
影响因子：
3.9
作者：
Lipsky, Zachary W.;German, Guy K.
通讯作者：
German, Guy K.
影响因子：
4.5
作者：
Mohammed, Diar;Matts, Paul J.;Lane, Majella E.
通讯作者：
Lane, Majella E.