Comparative transcriptomics and host-specific parasite gene expression profiles inform on drivers of proliferative kidney disease.
Comparative transcriptomics and host-specific parasite gene expression profiles inform on drivers of proliferative kidney disease.
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比较转录组学和宿主特异性寄生虫基因表达谱为增殖性肾病的驱动因素提供信息
DOI：
10.1038/s41598-020-77881-7
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发表时间：
2021-01-25
影响因子：
4.6
通讯作者：
Holland JW
中科院分区：
文献类型：
--
作者：
Faber M;Shaw S;Yoon S;de Paiva Alves E;Wang B;Qi Z;Okamura B;Hartikainen H;Secombes CJ;Holland JW
粘寄生虫，Tetracapsuloides bryosalmonae有一个两个主机之间的生活周期交替淡水苔藓虫和鲑鱼。受感染的鱼可能会发展为肾脏疾病，其特征是野生和养殖鲑鱼中淋巴细胞驱动的肾脏病理。为了便于深入了解T.为了研究苔藓虫-宿主相互作用，我们使用了双宿主寄生虫转录组测序方法来产生两种寄生虫转录组组装体;第一种来源于从感染的苔藓虫分离的寄生虫孢子囊，第二种来源于感染的鱼肾组织。采用这种方法是为了在没有完整T.苔藓鲑基因组。两种感染宿主共有的寄生虫重叠群（交叉转录组; 7362个重叠群）通常富含AT（60-75% AT）。注释了intersect内的5432个重叠群。1930个未注释的重叠群编码未知的转录本。我们专注于转录编码蛋白质参与;营养获取，宿主-寄生虫相互作用，发育，细胞间通讯和未知功能的蛋白质，通过RT-qPCR建立它们在每个宿主中的潜在重要性。宿主特异性表达谱是明显的，特别是在转录编码蛋白酶和蛋白质参与脂质代谢，细胞粘附和发展。我们首次证实了粘虫寄生虫中同源异型盒蛋白和卷曲同源物的存在。这项研究揭示的对粘虫生物学的新见解将有助于将研究重点放在制定未来的疾病控制策略上。
The myxozoan parasite, Tetracapsuloides bryosalmonae has a two-host life cycle alternating between freshwater bryozoans and salmonid fish. Infected fish can develop Proliferative Kidney Disease, characterised by a gross lymphoid-driven kidney pathology in wild and farmed salmonids. To facilitate an in-depth understanding of T. bryosalmonae-host interactions, we have used a two-host parasite transcriptome sequencing approach in generating two parasite transcriptome assemblies; the first derived from parasite spore sacs isolated from infected bryozoans and the second from infected fish kidney tissues. This approach was adopted to minimize host contamination in the absence of a complete T. bryosalmonae genome. Parasite contigs common to both infected hosts (the intersect transcriptome; 7362 contigs) were typically AT-rich (60–75% AT). 5432 contigs within the intersect were annotated. 1930 unannotated contigs encoded for unknown transcripts. We have focused on transcripts encoding proteins involved in; nutrient acquisition, host–parasite interactions, development, cell-to-cell communication and proteins of unknown function, establishing their potential importance in each host by RT-qPCR. Host-specific expression profiles were evident, particularly in transcripts encoding proteases and proteins involved in lipid metabolism, cell adhesion, and development. We confirm for the first time the presence of homeobox proteins and a frizzled homologue in myxozoan parasites. The novel insights into myxozoan biology that this study reveals will help to focus research in developing future disease control strategies.
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影响因子：
16.6
作者：
Berthelot, Camille;Brunet, Frederic;Chalopin, Domitille;Juanchich, Amelie;Bernard, Maria;Noel, Benjamin;Bento, Pascal;Da Silva, Corinne;Labadie, Karine;Alberti, Adriana;Aury, Jean-Marc;Louis, Alexandra;Dehais, Patrice;Bardou, Philippe;Montfort, Jerome;Klopp, Christophe;Cabau, Cedric;Gaspin, Christine;Thorgaard, Gary H.;Boussaha, Mekki;Quillet, Edwige;Guyomard, Rene;Galiana, Delphine;Bobe, Julien;Volff, Jean-Nicolas;Genet, Carine;Wincker, Patrick;Jaillon, Olivier;Roest Crollius, Hugues;Guiguen, Yann
通讯作者：
Guiguen, Yann
影响因子：
1.3
作者：
Foox, Jonathan;Siddall, Mark E.
通讯作者：
Siddall, Mark E.
DOI：
10.1073/pnas.1819976116
发表时间：
2019-04-02
影响因子：
11.1
作者：
Dorrell, Richard G.;Azuma, Tomonori;Kamikawa, Ryoma
通讯作者：
Kamikawa, Ryoma
影响因子：
2.9
作者：
Bailey, Christyn;Strepparava, Nicole;Segner, Helmut
通讯作者：
Segner, Helmut
影响因子：
3.1
作者：
Bai, Zhiyi;Lin, Jingyun;Li, Jiale
通讯作者：
Li, Jiale