The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway.
The silencing of Pokemon attenuates the proliferation of hepatocellular carcinoma cells in vitro and in vivo by inhibiting the PI3K/Akt pathway.
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口袋妖怪的沉默通过抑制PI3K/AKT途径来减轻体外和体内肝细胞癌细胞的增殖。
DOI：
10.1371/journal.pone.0051916
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ren JL
中科院分区：
文献类型：
--
作者：
Lin CC;Zhou JP;Liu YP;Liu JJ;Yang XN;Jazag A;Zhang ZP;Guleng B;Ren JL
Pokemon （POK erythroid myeloid ontogenic factor）属于POK蛋白家族，又称LRF、OCZF和FBI-1。作为一种转录抑制因子，Pokemon在细胞分化和肿瘤发生中起着关键作用。我们的研究确定了Pokemon在人类肝细胞癌（HCC）中的致癌作用。我们成功地建立了人类HepG2和Huh-7细胞系，在这些细胞系中，Pokemon被稳定地击倒。我们证明了Pokemon沉默可以抑制细胞增殖和迁移。Pokemon敲除抑制了PI3K/Akt和c-Raf/MEK/ERK通路，并调节了HepG2和Huh-7细胞中各种细胞周期调节因子的表达。因此，Pokemon也可能参与了这些细胞的细胞周期进程。我们证实了Pokemon沉默抑制肿瘤移植小鼠的肝细胞癌生长。这些结果表明，Pokemon在肝细胞癌中促进细胞增殖和迁移，并以Akt和erk信号依赖的方式加速肿瘤发展。
Pokemon (POK erythroid myeloid ontogenic factor), which belongs to the POK protein family, is also called LRF, OCZF and FBI-1. As a transcriptional repressor, Pokemon assumes a critical function in cellular differentiation and oncogenesis. Our study identified an oncogenic role for Pokemon in human hepatocellular carcinoma (HCC). We successfully established human HepG2 and Huh-7 cell lines in which Pokemon was stably knocked down. We demonstrated that Pokemon silencing inhibited cell proliferation and migration. Pokemon knockdown inhibited the PI3K/Akt and c-Raf/MEK/ERK pathways and modulated the expression of various cell cycle regulators in HepG2 and Huh-7 cells. Therefore, Pokemon may also be involved in cell cycle progression in these cells. We confirmed that Pokemon silencing suppresses hepatocellular carcinoma growth in tumor xenograft mice. These results suggest that Pokemon promotes cell proliferation and migration in hepatocellular carcinoma and accelerates tumor development in an Akt- and ERK-signaling-dependent manner.
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