Circulating fibrocyte mobilization in negative pressure wound therapy.
Circulating fibrocyte mobilization in negative pressure wound therapy.
复制标题
负压伤口治疗中的循环纤维细胞动员
DOI：
10.1111/jcmm.13080
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发表时间：
2017-08
影响因子：
5.3
通讯作者：
Yu A
中科院分区：
文献类型：
--
作者：
Chen D;Zhao Y;Li Z;Shou K;Zheng X;Li P;Qi B;Yu A
不愈合的糖尿病伤口很难治疗。它们也给患者和社会带来了沉重的经济负担。负压创面疗法（NPWT）已被应用于治疗顽固性创面，并已被证明是有效的。然而，这种治疗效果背后的机制尚不完全清楚。循环纤维细胞是一种独特的造血干细胞，在伤口愈合中起着关键作用。在这里，我们研究了NPWT对纤维细胞动员的影响，以及NPWT期间纤维细胞动员在糖尿病伤口愈合中的作用。我们发现，与对照组相比，NPWT组在糖尿病伤口部位显示出2.6 - 12.1倍的尾静脉注射PKH - 26标记的纤维细胞。我们还证明，当外源性纤维细胞动员受到抑制时，NPWT处理的全层皮肤伤口的mRNA和蛋白表达、血管密度和增殖细胞显著降低。我们推测，在糖尿病大鼠模型实验中，NPWT期间纤维细胞的全身动员可能是治疗难治性伤口的一种机制，增强细胞动员可能代表了所有手术领域难治性伤口愈合的潜在治疗理念。
Non‐healing diabetic wounds are difficult to treat. They also create heavy financial burdens for both patients and society. Negative pressure wound therapy (NPWT) has been adopted to treat intractable wounds and has proved to be effective. However, the mechanisms that underlie the effects of this treatment are not entirely understood. Circulating fibrocytes are unique haematopoietic‐derived stem cells that have been reported to play a pivotal role in wound healing. Here, we have investigated the effect of NPWT on fibrocyte mobilization and the role of fibrocyte mobilization in the healing of diabetic wounds during NPWT. We show that the NPWT group exhibited 2.6‐fold to 12.1‐fold greater numbers of tail vein‐injected PKH‐26‐labelled fibrocytes in the diabetic wound sites compared with the control group. We also demonstrate that the full‐thickness skin wounds treated with NPWT exhibit significantly reduced mRNA and protein expression, blood vessel density and proliferating cells when exogenous fibrocyte mobilization is inhibited. We speculate that systemic mobilization of fibrocytes during NPWT may be a mechanism for healing intractable wounds in a diabetic rat model experiment and that enhancement of cell mobilization may represent a potential treatment idea for intractable wound healing across all fields of surgery.
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影响因子：
15.9
作者：
LYNCH, SE;COLVIN, RB;ANTONIADES, HN
通讯作者：
ANTONIADES, HN
影响因子：
4.3
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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