ARGONAUTE10 Inhibits In Vitro Shoot Regeneration Via Repression of miR165/166 in Arabidopsis thaliana
ARGONAUTE10 Inhibits In Vitro Shoot Regeneration Via Repression of miR165/166 in Arabidopsis thaliana
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ARGONAUTE10 通过抑制拟南芥中的 miR165/166 来抑制体外芽再生
DOI：
10.1093/pcp/pcx117
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发表时间：
2017-10
影响因子：
4.9
通讯作者：
Xiang Fengning
中科院分区：
文献类型：
--
作者：
Xue Tao;Dai Xuehuan;Wang Ruipu;Wang Junzhu;Liu Zhenhua;Xiang Fengning
许多植物细胞在体外培养时仍保持其全能性。体外培养对芽再生的调控涉及到许多基因产物，但相关转录后事件的性质在很大程度上仍不清楚。在这里，转录后调节因子ARGONAUTE10(AGO10)是一种在原茎顶端分生组织(SAM)形成期间在外植体中特异表达的蛋白质，已知它能抑制不定芽再生。在功能丧失突变体ago10的体外培养外植体中，形成了比正常数量多得多的SAM，其中干细胞标记基因WUSCHEL、CLAVATA3和芽MERISTEMLESS都得到了强烈的表达。AGO10抑制了microRNAs miR165/166的积累，从而上调了一套HD-ZIP III基因。MiR166的过量表达促进了不定芽的再生，而miR165/166信息的缺失则阻碍了不定芽的再生，仅部分挽救了ago10突变体的表型。主要结论是AGO10抑制不定芽再生是通过抑制miR165/166来实现的。
Many plant cells retain their totipotency when cultured in vitro. The regulation of shoot regeneration from in vitro culture involves a number of gene products, but the nature of the associated post-transcriptional events remains largely unknown. Here, the post-transcriptional regulator ARGONAUTE10 (AGO10), a protein which is specifically expressed in the explant during the period when pro-shoot apical meristems (SAMs) are forming, has been known to inhibit shoot regeneration. In in vitro cultured explants of the loss-of-function mutant ago10, a much larger than normal number of SAMs was formed and, in these, the stem cell marker genes WUSCHEL, CLAVATA3 and SHOOT MERISTEMLESS were all strongly expressed. AGO10 repressed the accumulation of the microRNAs miR165/166, thereby up-regulating a suite of HD-ZIP III genes. The overproduction of miR166 was shown to promote shoot regeneration, while the absence of miR165/166 message resulted in a blockage to shoot regeneration and only a partial rescue of the phenotype of the ago10 mutant. The major conclusion was that the shoot regeneration inhibition determined by AGO10 functions via the repression of miR165/166.
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