Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection.
Multilayer Heterogeneous Membrane Biosensor Based on Multiphysical Field Coupling for Human Serum Albumin Detection.
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DOI：
10.1021/acsomega.2c07338
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发表时间：
2023-01-24
期刊：
影响因子：
4.1
通讯作者：
Zhao, Dong
中科院分区：
文献类型：
--
作者：
Wang, Haoyu;Xiao, Pengli;Sang, Shengbo;Chen, Honglie;Dong, Xiushan;Ge, Yang;Guo, Xing;Zhao, Dong
临床诊断中与肾脏疾病状态密切相关的一个因素是人体体液尿液、血清等中人血清白蛋白（HSA）浓度水平异常。表面应力生物传感器作为检测蛋白质分子浓度的新型生物传感器而发展起来，具有广泛的临床应用。然而，需要进一步提高灵敏度以实现更高的检测性能。在此，成功开发了基于磁场、电场和表面应力场耦合的MXene/PDMS/Fe3O4/PDMS多层异质膜生物传感器（MHBios），通过磁敏化实现高灵敏度HSA检测。修饰后的抗体与 AuNP 层的 HSA 特异性结合，使生物传感器能够将 PDMS 薄膜变形引起的表面应力转化为电信号。当生物传感器暴露在均匀磁场中时，由于磁力放大了PDMS薄膜的变形，导电层的导电路径进一步重塑，增强了生物信号到电信号的转换。结果显示，当HSA浓度为0~50 μg/mL时，MHBios的检出限（LOD）为78 ng/mL，明显低于微量白蛋白尿的诊断下限。此外，MHBios 在实际样本中检测到了 HSA，证实了早期疾病筛查的潜力。
A factor closely associated with renal disease status in clinical diagnosis is abnormal human serum albumin (HSA) concentration levels in human body fluids urine, serum, etc. The surface stress biosensor was developed as a new type of biosensor to detect protein molecule concentration and has a wide range of clinical applications. However, further sensitivity improvement is required to achieve higher detection performance. Herein, MXene/PDMS/Fe3O4/PDMS of the multilayer heterogeneous membrane biosensor (MHBios) based on the coupling of the magnetic field, electric field, and surface stress field was successfully developed to achieve high sensitivity HSA detection through magnetic sensitization. The modified antibody specifically binds to HSA at the AuNP layer, allowing the biosensor to convert the surface stress caused by PDMS film deformation into an electrical signal. When the biosensor was exposed to a uniform magnetic field, the conductive path of the conductive layer was reshaped further as the magnetic force amplified the deformation of the PDMS film, enhancing the conversion of biological signals to electrical signals. The results exhibited that the detection limit (LOD) of the MHBios was 78 ng/mL when HSA concentration was 0–50 μg/mL, which was markedly lower than the minimum diagnostic limit of microalbuminuria. Furthermore, the MHBios detected HSA in actual samples, confirming the potential for early disease screening.
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