Anti-inflammatory actions of endogenous and exogenous interleukin-10 versus glucocorticoids on macrophage functions of the newly born.
Anti-inflammatory actions of endogenous and exogenous interleukin-10 versus glucocorticoids on macrophage functions of the newly born.
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DOI：
10.1038/jp.2014.16
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发表时间：
2014-05
影响因子：
2.9
通讯作者：
Davidson, D.
中科院分区：
文献类型：
--
作者：
Kasat, K.;Patel, H.;Predtechenska, O.;Vancurova, I.;Davidson, D.
为了确定新生儿的特异性巨噬细胞免疫功能是否对治疗水平的地塞米松（DEX）不敏感，之前在支气管肺发育不良（BPD）婴儿中测量，与倍他米松（BETA）和外源性或内源性白细胞介素-10 （IL-10）相比。巨噬细胞从脐带血单核细胞分化而来（N=18）。采用连续剂量效应（约10−8M），体外研究DEX、BETA和IL-10对促炎（PI）细胞因子释放、吞噬和呼吸爆发的影响。外源性IL-10（10−8M）显著（p<0.05）抑制内毒素刺激的IL-6、IL-8和肿瘤坏死因子的释放，抑制率为63% ~ 82%，而DEX和BETA对内毒素刺激的IL-6、IL-8和肿瘤坏死因子的释放无显著影响。在10−8M时，3种药物对大鼠的吞噬和呼吸爆发均无抑制作用。单克隆抗体抑制内源性IL-10显著提高内毒素刺激的细胞因子释放至少4倍。巨噬细胞对治疗水平的DEX和BETA的PI细胞因子释放相对不敏感。本研究为应用气道注入IL-10治疗BPD的临床前研究提供了理论依据。
To determine whether specific macrophage immune functions of the newly born are insensitive to the actions of therapeutic levels of dexamethasone (DEX), previously measured in infants with bronchopulmonary dysplasia (BPD), compared to betamethasone (BETA) and exogenous or endogenous interleukin-10 (IL-10). Macrophages were differentiated from cord blood monocytes (N=18). A serial dose response (around 10−8M), in vitro study was used to examine the effect of DEX, BETA and IL-10, on pro-inflammatory (PI) cytokine release, phagocytosis and respiratory burst. Exogenous IL-10 (10−8M) significantly (p<0.05) inhibited the endotoxin-stimulated release of IL-6, IL-8 and tumor necrosis factor by 63% to 82% with no significant effect by DEX and BETA. There was no inhibition by these 3 agents at 10−8M on phagocytosis and respiratory burst. Inhibition of endogenous IL-10 with a monoclonal antibody significantly raised endotoxin-stimulated cytokine release by at least 4 fold. Macrophages were relatively insensitive to therapeutic levels of DEX and BETA with regard to PI cytokine release. This study provides rationale for translational, preclinical research using airway instillation of IL-10 for the treatment of BPD.
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影响因子：
2.5
作者：
Chusid, Lina A.;Pereira-Argenziano, Lucy;Davidson, Dennis
通讯作者：
Davidson, Dennis
影响因子：
158.5
作者：
Clark, RH;Kueser, TJ;Kinsella, JP
通讯作者：
Kinsella, JP
DOI：
10.1016/0002-9378(88)90216-5
发表时间：
1988-05-01
影响因子：
9.8
作者：
ROMERO, R;ROSLANSKY, P;HOBBINS, JC
通讯作者：
HOBBINS, JC
DOI：
10.1152/ajplung.00251.2009
发表时间：
2010-01-01
影响因子：
4.9
作者：
Mokres, Lucia M.;Parai, Kakoli;Bland, Richard D.
通讯作者：
Bland, Richard D.
影响因子：
3.6
作者：
Jobe AH
通讯作者：
Jobe AH