Prediction of lymph node metastasis in oral squamous cell carcinoma based on protein profile
Prediction of lymph node metastasis in oral squamous cell carcinoma based on protein profile
复制标题
基于蛋白质谱预测口腔鳞癌淋巴结转移
DOI：
10.1080/14789450.2019.1584039
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发表时间：
2019-03
影响因子：
3.4
通讯作者：
Chen Wantao
中科院分区：
文献类型：
--
作者：
Yu Binbin;Cao Wei;Zhang Chenping;Xia Ronghui;Liu Jinlin;Yan Ming;Chen Wantao
摘要目的：淋巴结转移是口腔鳞状细胞癌（OSCC）高死亡率的主要原因。但临床阴性颈（cN 0）和阳性颈（cN 1 -3）的定义仍有争议。方法：我们从已发表的文献中检索通过蛋白质组学分析鉴定的候选生物标志物。在训练阶段，采用免疫组化（IHC）分析蛋白质的表达，并采用逐步变量选择的Logistic回归模型分析可能影响淋巴结转移和生活状态的潜在因素。在验证阶段对预测模型进行了验证。结果如下：我们筛选了8个与OSCC淋巴结转移相关的高表达蛋白，发现SOD 2、BST 2、CAD、ITGB 6和PRDX 4在有淋巴结转移的患者中的表达水平显著高于无淋巴结转移的患者。此外，在训练和验证阶段，开发了基于CAD、SOD 2表达水平和组织病理学分级的组合的预测模型，并在OSCC患者中进行了验证。结论：我们的研究结果显示，所发展的模型可以很好地预测口腔鳞状细胞癌患者的淋巴结转移和生活状况，而不依赖于TNM分期。
ABSTRACT Objective: Lymph node metastasis leads to high mortality rates of oral squamous cell carcinoma (OSCC). However, it is still controversial to define clinically negative neck (cN0) and positive neck (cN1-3). Methods: We retrieved candidate biomarkers identified by proteomic analysis in OSCC from published works of literature. In training stage, immunohistochemistry (IHC) analysis was used to determine the expression of proteins and logistic regression models with stepwise variable selection were used to identify potential factors that might affect lymph node metastasis and life status. Furthermore, the prediction model was validated in validating stage. Results: We screened eight highly expressed proteins related to lymph node metastasis in OSCC and found that the expression levels of SOD2, BST2, CAD, ITGB6, and PRDX4 were significantly elevated in patients with lymph node metastasis compared to the patients without lymph node metastasis. Furthermore, in training and validating stages, the prediction model base on the combination of CAD, SOD2 expression levels, and histopathologic grade was developed and validated in patients with OSCC. Conclusions: Our findings showed that the developed model well predicts the lymph node metastasis and life status in patients with OSCC, independent of TNM stage.
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DOI：
10.4103/0973-029x.119740
发表时间：
2013-05
期刊：
Journal of oral and maxillofacial pathology : JOMFP
影响因子：
--
作者：
Sharma M;Sah P;Sharma SS;Radhakrishnan R
通讯作者：
Radhakrishnan R
影响因子：
6.2
作者：
Chang, Kai-Ping;Wang, Chih-Lueh Albert;Chi, Lang-Ming
通讯作者：
Chi, Lang-Ming
影响因子：
5.6
作者：
Brandwein-Gensler, Margaret;Teixeira, Miriam S.;Wang, Beverly Yiyao
通讯作者：
Wang, Beverly Yiyao
影响因子：
7
作者：
Chen, Yi-Ting;Chen, Hsiao-Wei;Yang, Wei-Ting Ou
通讯作者：
Yang, Wei-Ting Ou
DOI：
10.4103/0970-9290.123385
发表时间：
2013-09
期刊：
Indian journal of dental research : official publication of Indian Society for Dental Research
影响因子：
--
作者：
S. Acharya;A. T. Sivakumar;S. Shetty
通讯作者：
S. Acharya;A. T. Sivakumar;S. Shetty