Mechanism of Azalomycin F(5a) against Methicillin-Resistant Staphylococcus aureus.
Mechanism of Azalomycin F(5a) against Methicillin-Resistant Staphylococcus aureus.
复制标题
阿扎霉素F5a对抗耐甲氧西林金黄色葡萄球菌的作用机制
DOI：
10.1155/2018/6942452
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发表时间：
2018
影响因子：
--
通讯作者：
Liu E
中科院分区：
文献类型：
--
作者：
Xu L;Xu X;Yuan G;Wang Y;Qu Y;Liu E
为了探讨阿扎霉素F5a抗耐甲氧西林金黄色葡萄球菌(MRSA)的作用机制，测定了阿扎霉素F5a干预下MRSA悬浮液的电导率和MRSA培养物的腺苷酸激酶活性，与空白对照相比，腺苷酸激酶活性均显着升高。由此推断，阿扎霉素F5a可能通过增加渗透性而导致细胞物质渗漏，从而杀死MRSA。由于磷脂双层主要负责细胞膜通透性，因此通过确定阿扎霉素 F5a 与从测试 MRSA 中提取的细胞膜脂质或与 1,2-二棕榈酰-sn-甘油-3-磷酸甘油 (DPPG) 结合的抗 MRSA 活性，进一步研究阿扎霉素 F5a 与细胞膜脂质之间的相互作用，以寻找 MRSA 中可能的分子靶点细胞膜。结果表明，阿扎霉素F5a与膜脂或DPPG结合后，抗MRSA活性显着降低。这表明细胞膜脂质尤其是DPPG可能是阿扎霉素F5a抗MRSA的重要靶点。
To investigate the mechanism of azalomycin F5a against methicillin-resistant Staphylococcus aureus (MRSA), the conductivity of MRSA suspension and the adenylate kinase activity of MRSA culture were determined with the intervention of azalomycin F5a, which were significantly increased compared to those of blank controls. This inferred that azalomycin F5a could lead to the leakage of cellular substances possibly by increasing permeability to kill MRSA. As phospholipid bilayer was mainly responsible for cell-membrane permeability, the interaction between azalomycin F5a and cell-membrane lipids was further researched by determining the anti-MRSA activities of azalomycin F5a combined with cell-membrane lipids extracted from test MRSA or with 1,2-dipalmitoyl-sn-glycero-3-phospho-glycerol (DPPG) for possible molecular targets lying in MRSA cell-membrane. The results indicated that the anti-MRSA activity of azalomycin F5a remarkably decreased when it combined with membrane lipids or DPPG. This indicated that cell-membrane lipids especially DPPG might be important targets of azalomycin F5a against MRSA.
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影响因子：
11.8
作者：
Boucher, Helen W.;Talbot, George H.;Bartlett, John
通讯作者：
Bartlett, John
影响因子：
3.8
作者：
Yuan, Ganjun;Li, Peibo;Chen, Shanjun
通讯作者：
Chen, Shanjun
DOI：
10.3839/jksabc.2010.084
发表时间：
2010-10-01
影响因子：
--
作者：
Cheng, Jinhua;Yang, Seung Hwan;Suh, Joo-Won
通讯作者：
Suh, Joo-Won
影响因子：
5.2
作者：
Steenbergen, JN;Alder, J;Tally, FP
通讯作者：
Tally, FP
影响因子：
4.9
作者：
Jacobs, Anna C.;DiDone, Louis;Dunman, Paul M.
通讯作者：
Dunman, Paul M.