Differentiation of prostate cancer cells using flexible fluorescent polymers.

Differentiation of prostate cancer cells using flexible fluorescent polymers.
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DOI:
10.1021/ac202301k
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发表时间:
2012-01-03
影响因子:
7.4
通讯作者:
Mallik, Sanku
Mallik, Sanku
中科院分区:
化学1区
文献类型:
--
作者:
Scott, Michael D.;Dutta, Rinku;Haldar, Manas K.;Guo, Bin;Friesner, Daniel L.;Mallik, Sanku

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使用水溶性的,荧光的,灵活的聚合物,我们已经设计了一种新的方法来识别和分化的前列腺癌细胞。通过使用逐步线性判别分析,我们证明了在条件细胞培养基存在下的聚合物发射强度的差分调制可用于区分前列腺癌亚型和癌细胞与非癌细胞之间。条件细胞培养基的组成的差异可能导致聚合物的不同荧光光谱模式。这种体外方法可能为开发替代前列腺癌诊断和分型技术提供新的平台。
Using water soluble, fluorescent, flexible polymers, we have devised a novel methodology for identification and differentiation of prostate cancer cells. By using a step-wise linear discriminant analysis we demonstrate that the differential modulations of the polymer emission intensities in the presence of conditioned cell culture media can be used to distinguish between prostate cancer subtypes and between cancerous and non-cancer cells. The differences in the compositions of the conditioned cell culture media are likely contributing to different fluorescence spectral patterns of the polymers. This in vitro approach may provide a novel platform for the development of an alternative prostate cancer diagnostic and subtyping technique.
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