Altered serum levels of IL-33 in patients with advanced systolic chronic heart failure: correlation with oxidative stress.
Altered serum levels of IL-33 in patients with advanced systolic chronic heart failure: correlation with oxidative stress.
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晚期收缩期慢性心力衰竭患者血清 IL-33 水平的变化：与氧化应激的相关性
DOI：
10.1186/1479-5876-10-120
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发表时间：
2012-06-08
影响因子：
7.4
通讯作者：
Zhu LG
中科院分区：
文献类型：
--
作者：
Zhang HF;Xie SL;Chen YX;Mai JT;Wang JF;Zhu WL;Zhu LG
背景白细胞介素-33（IL-33）与慢性心力衰竭（CHF）的关系在动物实验中得到证实，但关于CHF患者血清IL-33水平的数据尚不清楚。我们分析了血清中IL-33的水平，并探讨了IL-33在氧化stress.MethodsA的可能作用，共191例晚期收缩期CHF（CHF组），175例预先存在的心脏疾病，但没有CHF（非CHF组），和177名健康对照（HC组）被招募。检测血清IL-33、可溶性ST 2（sST 2）、N末端脑钠肽前体（NT-proBNP）、丙二醛（MDA）含量、红细胞超氧化物歧化酶（eSOD）活性及左室射血分数（LVEF）。确定了血清中IL-33的确切形式。结果CHF患者血清IL-33和sST 2水平升高，IL-33/sST 2比值降低。在CHF患者中，既存心脏病和入院时使用的药物不影响IL-33浓度或IL-33/sST 2比值。全长IL-33在HC患者血清中检测不到，在非CHF患者中几乎检测不到，但在CHF患者中显著上调。IL-33水平与CHF严重程度标志物呈正相关。IL-33/sST 2比值与MDA浓度呈轻微负相关。结论CHF患者血清IL-33水平升高，尤其是全长IL-33水平升高，而IL-33活性降低。在晚期CHF中，IL-33可能发挥抗氧化作用，这可能被同时升高的sST 2水平所压倒。
BackgroundInterleukin-33 (IL-33) has been linked to chronic heart failure (CHF) in animal studies, but data on serum IL-33 levels in human CHF are not available. We analyzed levels of IL-33 in serum, and investigated the possible role of IL-33 in oxidative stress.MethodsA total of 191 subjects with advanced systolic CHF (CHF group), 175 patients with pre-existing cardiac diseases but no CHF (non-CHF group), and 177 healthy controls (HC group) were enrolled. Serum levels of IL-33, soluble ST2 (sST2) and N-terminal-pro-brain natriuretic peptide (NT-proBNP), malondialdehyde (MDA) content, erythrocyte superoxide dismutase (eSOD) activity, as well as left ventricular ejection fraction (LVEF), were determined. The exact form of IL-33 in serum was identified. Effects of IL-33 and sST2 on MDA content and SOD activity in angiotensin (Ang II)-stimulated AC16 cells were assessed.ResultsSerum levels of IL-33 and sST2 were elevated in CHF patients, whereas IL-33/sST2 ratios were decreased. In CHF patients, pre-existing cardiac diseases and medications used upon hospital admission did not affect IL-33 concentrations or the IL-33/sST2 ratio. Full-length IL-33, which could not be detected in serum from HC and barely detected in non-CHF patients, was significantly up-regulated in CHF patients. IL-33 levels were positively correlated with markers of CHF severity. IL-33/sST2 ratios were slightly and negatively related to MDA concentrations. IL-33 directly reduced MDA and enhanced SOD activity in Ang II-stimulated AC16 cells, which were greatly attenuated by sST2.ConclusionsSerum levels of IL-33, especially the full-length form, were elevated in CHF patients whereas IL-33 bioactivity was reduced. In advanced CHF, IL-33 may exert anti-oxidation effects, which may be overwhelmed by concurrently elevated levels of sST2.
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DOI：
10.1073/pnas.0812690106
发表时间：
2009-06-02
影响因子：
11.1
作者：
Cayrol, Corinne;Girard, Jean-Philippe
通讯作者：
Girard, Jean-Philippe
影响因子：
4.5
作者：
Hong YS;Moon SJ;Joo YB;Jeon CH;Cho ML;Ju JH;Oh HJ;Heo YJ;Park SH;Kim HY;Min JK
通讯作者：
Min JK
影响因子：
3.9
作者：
Mu, Rong;Huang, He-Qing;Li, Zhan-Guo
通讯作者：
Li, Zhan-Guo
影响因子：
120.7
作者：
Redfield, MM;Jacobsen, SJ;Rodeheffer, RJ
通讯作者：
Rodeheffer, RJ
影响因子：
15.9
作者：
Sanada, Shoji;Hakuno, Daihiko;Lee, Richard T.
通讯作者：
Lee, Richard T.