Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles.
Hemocyte Clusters Defined by scRNA-Seq in Bombyx mori: In Silico Analysis of Predicted Marker Genes and Implications for Potential Functional Roles.
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家蚕中 scRNA-Seq 定义的血细胞簇：预测标记基因的计算机分析及其对潜在功能作用的影响
DOI：
10.3389/fimmu.2022.852702
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发表时间：
2022
影响因子：
7.3
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Feng M;Swevers L;Sun J
在血淋巴中，昆虫血细胞构成了巨噬细胞样细胞的异质种群，在先天免疫，稳态和发育中起着重要作用。根据大小，形态和生化或免疫学标记的不同亚型中血细胞的分类非常困难，只有在果蝇广泛的遗传分析中，才允许构建从亲毛细胞到肉芽细胞，晶体细胞和血浆细胞，晶体细胞，晶体细胞和血浆细胞的相干分化的相干图片。然而，高通量单细胞技术的出现，例如单细胞RNA测序（SCRNA-SEQ），对血细胞亚型的研究及其在其他昆虫中的表型产生很高的影响，而这些昆虫的表型不可用。 SCRNA-Seq并没有在大量RNA-Seq中平均所有细胞中的基因表达，而是可以对单个细胞的分化状态进行高通量和特定的可视化。使用SCRNA-SEQ，可以在异质种群中鉴定有趣的细胞类型，并且可以直接分析稀有细胞类型。除了它介绍组织样品中单个细胞转录组的能力之后，SCRNA-SEQ可用于提出标记基因，这些标记基因是不同血细胞亚型的特征并预测其功能。从这个角度来看，通过详细讨论了通过SCRNA-SEQ分析鉴定的不同标记基因的身份，以详细讨论了蚕虫中的13个不同的细胞簇，Bombyx Mori。该分析证实了粒细胞，浆细胞，烯型细胞和可能球细胞中血细胞的广泛分裂，但在分子水平和高度专业的功能上也揭示了相当大的复杂性。此外，Bombyx中预测的血细胞标记基因通常仅显示与被认为是果蝇中血细胞亚型特征的基因的收敛有限。
Within the hemolymph, insect hemocytes constitute a heterogeneous population of macrophage-like cells that play important roles in innate immunity, homeostasis and development. Classification of hemocytes in different subtypes by size, morphology and biochemical or immunological markers has been difficult and only in Drosophila extensive genetic analysis allowed the construction of a coherent picture of hemocyte differentiation from pro-hemocytes to granulocytes, crystal cells and plasmatocytes. However, the advent of high-throughput single cell technologies, such as single cell RNA sequencing (scRNA-seq), is bound to have a high impact on the study of hemocytes subtypes and their phenotypes in other insects for which a sophisticated genetic toolbox is not available. Instead of averaging gene expression across all cells as occurs in bulk-RNA-seq, scRNA-seq allows high-throughput and specific visualization of the differentiation status of individual cells. With scRNA-seq, interesting cell types can be identified in heterogeneous populations and direct analysis of rare cell types is possible. Next to its ability to profile the transcriptomes of individual cells in tissue samples, scRNA-seq can be used to propose marker genes that are characteristic of different hemocyte subtypes and predict their functions. In this perspective, the identities of the different marker genes that were identified by scRNA-seq analysis to define 13 distinct cell clusters of hemocytes in larvae of the silkworm, Bombyx mori, are discussed in detail. The analysis confirms the broad division of hemocytes in granulocytes, plasmatocytes, oenocytoids and perhaps spherulocytes but also reveals considerable complexity at the molecular level and highly specialized functions. In addition, predicted hemocyte marker genes in Bombyx generally show only limited convergence with the genes that are considered characteristic for hemocyte subtypes in Drosophila.
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影响因子：
2
作者：
Rorick, Nicholas K.;Kinoshita, Akira;Weirather, Jason L.;Peyrard-Janvid, Myriam;Ferreira de Lima, Renata L. L.;Dunnwald, Martine;Shanske, Alan L.;Moretti-Ferreira, Danilo;Koillinen, Hannele;Kere, Juha;Mansilla, Maria A.;Murray, Jeffrey C.;Goudy, Steve L.;Schutte, Brian C.
通讯作者：
Schutte, Brian C.
影响因子：
2.9
作者：
An, Chunju;Ragan, Emily J.;Kanost, Michael R.
通讯作者：
Kanost, Michael R.
影响因子：
7.3
作者：
Angajala A;Lim S;Phillips JB;Kim JH;Yates C;You Z;Tan M
通讯作者：
Tan M
影响因子：
5.5
作者：
Cattenoz PB;Monticelli S;Pavlidaki A;Giangrande A
通讯作者：
Giangrande A
影响因子：
4.8
作者：
Ahmad, M;Srinivasula, SM;Alnemri, ES
通讯作者：
Alnemri, ES