Characterization of Dak Nong virus, an insect nidovirus isolated from Culex mosquitoes in Vietnam.

Characterization of Dak Nong virus, an insect nidovirus isolated from Culex mosquitoes in Vietnam.
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DOI:
10.1007/s00705-013-1741-4
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发表时间:
2013-11
影响因子:
2.7
通讯作者:
Sawabe K
Sawabe K
中科院分区:
医学4区
文献类型:
--
作者:
Kuwata R;Satho T;Isawa H;Yen NT;Phong TV;Nga PT;Kurashige T;Hiramatsu Y;Fukumitsu Y;Hoshino K;Sasaki T;Kobayashi M;Mizutani T;Sawabe K

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在这项研究中,我们从越南的三带喙库蚊(双翅目:蚊科)中分离出一种昆虫巢状病毒,作为巢状病毒目中新家族中的一员。该病毒被命名为“Dak Nong病毒(DKNV)”,与最近在蚊子中发现的Cavally病毒和南定病毒有许多共同特征,这些病毒应被视为单一病毒种Alphamesonivirus 1的成员。DKNV在培养的蚊子细胞中生长,但不能在测试的培养的脊椎动物细胞中复制。DKNV结构蛋白的N-末端测序揭示了刺突糖蛋白前体中的两个翻译后切割位点。DKNV被认为是Alphamesonivirus 1的新成员,目前的研究提供了对属于新家族Mesoniviridae的病毒的进一步了解。本文的在线版本(doi:10.1007/s 00705 -013-1741-4)包含补充材料,可供授权用户使用。
In this study, we isolated and characterized an insect nidovirus from the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae) in Vietnam, as an additional member of the new family Mesoniviridae in the order Nidovirales. The virus, designated “Dak Nong virus (DKNV),” shared many characteristics with Cavally virus and Nam Dinh virus, which have also been discovered recently in mosquitoes, and these viruses should be considered members of a single virus species, Alphamesonivirus 1. DKNV grew in cultured mosquito cells but could not replicate in the cultured vertebrate cells tested. N-terminal sequencing of the DKNV structural proteins revealed two posttranslational cleavage sites in the spike glycoprotein precursor. DKNV is assumed to be a new member of the species Alphamesonivirus 1, and the current study provides further understanding of viruses belonging to the new family Mesoniviridae. The online version of this article (doi:10.1007/s00705-013-1741-4) contains supplementary material, which is available to authorized users.
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