Expression analysis of the Islet-1 gene in the developing and adult gastrointestinal tract.
Expression analysis of the Islet-1 gene in the developing and adult gastrointestinal tract.
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DOI：
10.1016/j.gep.2010.12.007
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发表时间：
2011-03
期刊：
影响因子：
--
通讯作者：
May CL
中科院分区：
文献类型：
--
作者：
Das P;May CL
LIM同源结构域基因编码一个由富含半胱氨酸的蛋白/蛋白相互作用（Lin-11、il -1和Mec-3） LIM结构域和高度保守的dna结合结构域定义的蛋白家族。对几种生物体的研究表明，这些转录调节因子控制着胚胎发育的多个方面，并对几种人类疾病的发病机制负责。在这里，我们报告了胰岛-1 （isl1）在发育和成年小鼠胃肠道上皮中的表达。在胚胎日(E) 9.5-10.5时，首先在胃腹侧间质中检测到is -1表达，几天后在胃背侧间质中开始表达。在E13.5时首次在胃上皮中观察到is -1的表达，E14.5时仅限于胃的后半部分。在成熟胃中，仅在肠内分泌细胞亚群中检测到isl1的表达。此外，在E15.5时首次在肠上皮中检测到isl1的表达，并且仅限于成年小鼠的肠内分泌细胞亚群。这些表达分析表明，is -1可能在胃和肠细胞中具有早期广泛的作用，并在胃肠道上皮成熟肠内分泌亚型的终末分化和/或维持中起次要作用。
LIM-Homeodomain genes encode a family of proteins defined by the cysteine-rich protein/protein interacting (Lin-11, Isl-1, and Mec-3) LIM domain and a highly conserved DNA-binding domain. Studies in several organisms have shown that these transcriptional regulators control multiple aspects of embryonic development and are responsible for the pathogenesis of several human diseases. Here we report the expression of Islet-1 (Isl-1) in the gastrointestinal epithelium in developing and adult mice. At embryonic day (E) 9.5–10.5, Isl-1 expression was first detected in the ventral gastric mesenchyme, and expression in the dorsal mesenchyme initiated a few days later. Isl-1 expression was first observed in the gastric epithelium at E13.5 and at E14.5 was restricted to the posterior half of the stomach. In the mature stomach, Isl-1 expression was detected only in subsets of enteroendocrine cells. Furthermore, Isl-1 expression in the intestinal epithelium was first detected at E15.5 and was restricted to subpopulations of enteroendocrine cells in adult mice. These expression analyses suggest that Isl-1 might have an early broad role in stomach and intestinal cells and a secondary role in terminal differentiation and/or maintenance of mature enteroendocrine subtypes in the gastrointestinal epithelium.
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