Next-generation sequencing technologies and their application to the study and control of bacterial infections.
Next-generation sequencing technologies and their application to the study and control of bacterial infections.
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DOI：
10.1016/j.cmi.2017.10.013
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发表时间：
2018-04
期刊：
影响因子：
--
通讯作者：
Trees E
中科院分区：
文献类型：
--
作者：
Besser J;Carleton HA;Gerner-Smidt P;Lindsey RL;Trees E
随着下一代测序成本和效率的降低，该技术迅速引入临床和公共卫生实验室实践。本文综述了第一、第二和第三代测序技术的历史背景和原理，以及最常用的测序仪器的特点。同行评审文献、白色文件和会议报告。下一代测序技术有可能取代许多传统的微生物工作流程，为临床医生和公共卫生专家提供比迄今为止更多的可操作信息。提供了该技术的临床和公共卫生用途的例子。讨论了不同测序平台可比性的挑战。最后，将其与实验室管理和公共卫生监测系统相结合，并将其直接从临床标本（宏基因组学）进行测序的技术的未来方向可能会导致临床诊断和公共卫生监测的另一次根本性转变。
With the decreasing cost and efficiency of next generation sequencing, the technology is rapidly introduced into clinical and public health laboratory practice. In this review, the historical background and principles of first, second and third generation sequencing are described as are the characteristics of the most commonly used sequencing instruments. Peer reviewed literature, white papers and meeting reports. Next generation sequencing is a technology that potentially could replace many traditional microbiological workflows, providing clinicians and public health specialists with more actionable information than hitherto achievable. Examples of the clinical and public health uses of the technology are provided. The challenge of comparability of different sequencing platforms is discussed. Finally, the future directions of the technology integrating it with laboratory management and public health surveillance systems, and moving it towards performing sequencing directly from the clinical specimen (metagenomics) could lead to yet another fundamental transformation of clinical diagnostics and public health surveillance.
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影响因子：
3.7
作者：
Cosentino S;Voldby Larsen M;Møller Aarestrup F;Lund O
通讯作者：
Lund O
实时全基因组测序，用于法国单核细胞增生李斯特菌的监视。
影响因子：
11.8
作者：
Moura A;Tourdjman M;Leclercq A;Hamelin E;Laurent E;Fredriksen N;Van Cauteren D;Bracq-Dieye H;Thouvenot P;Vales G;Tessaud-Rita N;Maury MM;Alexandru A;Criscuolo A;Quevillon E;Donguy MP;Enouf V;de Valk H;Brisse S;Lecuit M
通讯作者：
Lecuit M
影响因子：
56.9
作者：
FLEISCHMANN, RD;ADAMS, MD;VENTER, JC
通讯作者：
VENTER, JC
影响因子：
9.4
作者：
Joensen, Katrine Grimstrup;Scheutz, Flemming;Aarestrup, Frank M.
通讯作者：
Aarestrup, Frank M.
DOI：
10.1016/j.diagmicrobio.2015.12.003
发表时间：
2016-04
影响因子：
2.9
作者：
Kingry LC;Rowe LA;Respicio-Kingry LB;Beard CB;Schriefer ME;Petersen JM
通讯作者：
Petersen JM