Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load.
Immature articular cartilage and subchondral bone covered by menisci are potentially susceptive to mechanical load.
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DOI：
10.1186/1471-2474-15-101
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发表时间：
2014-03-26
影响因子：
2.3
通讯作者：
Kuroki H
中科院分区：
文献类型：
--
作者：
Iijima H;Aoyama T;Ito A;Tajino J;Nagai M;Zhang X;Yamaguchi S;Akiyama H;Kuroki H
半月板覆盖软骨和未覆盖软骨之间的力学和组织学特性的差异可能导致半月板切除术后骨关节炎的发生，这些差异尚不完全清楚。本研究的目的是研究未成熟膝关节半月板覆盖和未覆盖区域之间的浅软骨层和软骨下骨的力学和组织学特性，特别是胶原结构的潜在差异。从半月板覆盖或未覆盖的猪胫骨软骨中获得骨软骨塞。研究人员对软骨的厚度、力学性能、组织学和含水量进行了调查，并对软骨下骨进行了显微计算机断层扫描分析，以比较这些区域。用扫描电镜观察胶原蛋白的结构。与半月板覆盖的软骨相比，半月板覆盖的软骨更薄，对压缩载荷的畸形程度更高，含水量更高。在未覆盖区域的软骨表层，胶原纤维密度较高，而在覆盖区域胶原纤维密度较低。此外，两个区域的软骨下骨结构不同，覆盖区域骨密度低。半月板覆盖的软骨与未覆盖的软骨在力学和组织学特性上都有所不同，特别是在表面胶原蛋白层的密度方面。这些区域差异可能与正常情况下局部力学环境有关，表明半月板覆盖的软骨受到半月板严密保护，不受极端力学载荷的影响。我们的研究结果表明，半月板切除术后覆盖区在极端直接的机械负荷下容易发生未成熟软骨退变和软骨下微骨折。
The differences of mechanical and histological properties between cartilage covered by menisci and uncovered by menisci may contribute to the osteoarthritis after meniscectomy and these differences are not fully understood. The purpose of this study is to investigate potential differences in the mechanical and histological properties, and in particular the collagen architecture, of the superficial cartilage layer and subchondral bone between regions covered and uncovered by menisci using immature knee. Osteochondral plugs were obtained from porcine tibial cartilage that was either covered or uncovered by menisci. Investigation of the thickness, mechanical properties, histology, and water content of the cartilage as well as micro-computed tomography analysis of the subchondral bone was performed to compare these regions. Collagen architecture was also assessed by using scanning electron microscopy. Compared to the cartilage uncovered by menisci, that covered by menisci was thinner and showed a higher deformity to compression loading and higher water content. In the superficial layer of cartilage in the uncovered regions, collagen fibers showed high density, whereas they showed low density in covered regions. Furthermore, subchondral bone architecture varied between the 2 regions, and showed low bone density in covered regions. Cartilage covered by menisci differed from that uncovered in both its mechanical and histological properties, especially with regards to the density of the superficial collagen layer. These regional differences may be related to local mechanical environment in normal condition and indicate that cartilage covered by menisci is tightly guarded by menisci from extreme mechanical loading. Our results indicate that immature cartilage degeneration and subchondral microfracture may occur easily to extreme direct mechanical loading in covered region after meniscectomy.
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影响因子：
2.4
作者：
Julkunen, Petro;Kiviranta, Panu;Korhonen, Rarni K.
通讯作者：
Korhonen, Rarni K.
影响因子：
7
作者：
Intema, F.;Hazewinkel, H. A. W.;Mastbergen, S. C.
通讯作者：
Mastbergen, S. C.
影响因子：
4.9
作者：
Bellido M;Lugo L;Roman-Blas JA;Castañeda S;Caeiro JR;Dapia S;Calvo E;Largo R;Herrero-Beaumont G
通讯作者：
Herrero-Beaumont G
影响因子：
7
作者：
Cake, M. A.;Read, R. A.;Little, C. B.
通讯作者：
Little, C. B.
影响因子：
2.4
作者：
Julkunen, Petro;Halmesmaki, Esa P.;Helminen, Heikki J.
通讯作者：
Helminen, Heikki J.