Pathogen infection induces specific transgenerational modifications to gene expression and fitness in Caenorhabditis elegans.
Pathogen infection induces specific transgenerational modifications to gene expression and fitness in Caenorhabditis elegans.
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DOI：
10.3389/fphys.2023.1225858
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发表时间：
2023
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
病原体在亲代的感染如何通过表观遗传修饰影响后代对相同病原体的反应一直是最近研究的主题。这些研究集中在归因于跨代表观遗传的变化，以及这些变化如何导致人群中行为或免疫反应的可观察差异。然而，我们质疑病原体感染是否会导致隐藏的表观遗传变化，这些变化在种群水平上是不可观察的。使用线虫秀丽隐杆线虫作为模式生物，我们研究了一代又一代的生存差异的未暴露和引发谱系的动物对人类机会致病菌肠道沙门氏菌。我们发现训练C.线虫对特定病原体的抵抗不会导致总体存活率的显著变化，而是缩小了世代之间的存活差异。基因表达的定量显示TFEB脂肪吞噬途径的特定成员的变化减少。我们还提供了第一份报告的重复模式的生存时间超过12代的过程中，控制谱系的C。优雅的。这种重复的模式表明，控制谱系的世代之间的存活率的变化不是随机的，但可能是由未知的机制进行调节。总的来说，我们的研究表明，病原体感染可以引起特定的表型变化，由于表观遗传修饰，和一个可能的系统的表观遗传调控之间的世代。
How pathogen infection in a parental generation affects response in future generations to the same pathogen via epigenetic modifications has been the topic of recent studies. These studies focused on changes attributed to transgenerational epigenetic inheritance and how these changes cause an observable difference in behavior or immune response in a population. However, we questioned if pathogen infection causes hidden epigenetic changes to fitness that are not observable at the population level. Using the nematode Caenorhabditis elegans as a model organism, we examined the generation-to-generation differences in survival of both an unexposed and primed lineage of animals against a human opportunistic pathogen Salmonella enterica. We discovered that training a lineage of C. elegans against a specific pathogen does not cause a significant change to overall survival, but rather narrows survival variability between generations. Quantification of gene expression revealed reduced variation of a specific member of the TFEB lipophagic pathway. We also provided the first report of a repeating pattern of survival times over the course of 12 generations in the control lineage of C. elegans. This repeating pattern indicates that the variability in survival between generations of the control lineage is not random but may be regulated by unknown mechanisms. Overall, our study indicates that pathogen infection can cause specific phenotypic changes due to epigenetic modifications, and a possible system of epigenetic regulation between generations.
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影响因子：
64.5
作者：
Ashe A;Sapetschnig A;Weick EM;Mitchell J;Bagijn MP;Cording AC;Doebley AL;Goldstein LD;Lehrbach NJ;Le Pen J;Pintacuda G;Sakaguchi A;Sarkies P;Ahmed S;Miska EA
通讯作者：
Miska EA
DOI：
10.1073/pnas.1904828116
发表时间：
2019-10-08
影响因子：
11.1
作者：
Ferro, Kevin;Peuss, Robert;Kurtz, Joachim
通讯作者：
Kurtz, Joachim
影响因子：
30.3
作者：
Anyanful A;Easley KA;Benian GM;Kalman D
通讯作者：
Kalman D
影响因子：
3.3
作者：
Jobson, Meghan A.;Jordan, James M.;Baugh, L. Ryan
通讯作者：
Baugh, L. Ryan
影响因子：
64.8
作者：
Lu, R;Maduro, M;Ding, SW
通讯作者：
Ding, SW