Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space.
Ferric quinate (QPLEX) interacts with the major outer membrane protein (MOMP) of Campylobacter jejuni and enters through the porin channel into the periplasmic space.
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DOI：
10.1016/j.csbj.2022.09.032
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发表时间：
2022
影响因子：
6
通讯作者：
Soultanas, Panos
中科院分区：
文献类型：
--
作者：
Okoye, Jennifer C.;Bellamy-Carter, Jeddidiah;Oldham, Neil J.;Oldfield, Neil J.;Mahdavi, Jafar;Soultanas, Panos
酪氨酸铁(TYPLEX)和亲缘关系密切的奎宁酸铁(QPLEX)等铁络合物是细菌铁载体的结构模拟。TYPLEX已作为一种饲料添加剂在商业肉鸡养殖中进行了试验，将弯曲菌负荷减少了2-3log10，并导致更快的生长和更好的饲料消耗。这些铁络合物提供了抗生素的良好替代饲料添加剂，有助于减少在肉鸡养殖中为控制弯曲杆菌感染而不加选择地使用预防性抗生素。在本研究中，我们发现QPLEX与空肠弯曲菌NCTC11168的主要外膜蛋白(MOMP)结合。MOMP是细菌表面的一种必需且丰富的外膜孔蛋白，作为粘附素通过介导空肠弯曲菌附着在肉鸡的肠道上皮而帮助建立感染。利用卡宾足迹，我们绘制了MOMP-QPLEX的相互作用图，并在电子对接中用互补的方式表明，QPLEX通过胞外面的相互作用进入孔蛋白通道，通过偶极横向电场向另一端向下移位，并在MOMP的胞内面释放到周质中。我们的研究提示了这些铁络合物非抗生素抗弯曲菌活性的潜在机制。
Ferric chelates like ferric tyrosinate (TYPLEX) and the closely related ferric quinate (QPLEX) are structural mimics of bacterial siderophores. TYPLEX has been trialled as a feed additive in farming of commercial broilers, reducing Campylobacter loads by 2–3 log10 and leading to faster growth and better feed consumption. These ferric chelates offer a good alternative feed additive to antibiotics helping to reduce the indiscriminate use of preventative antibiotics in broiler farming to control Campylobacter infections. In this study, we show that QPLEX binds to the Major Outer Membrane Protein (MOMP) of C. jejuni NCTC11168. MOMP is an essential and abundant outer membrane porin on the surface of the bacteria, acting as an adhesin to help establish infection by mediating attachment of C. jejuni onto the gut epithelium of broilers and establish infection. Using carbene footprinting, we map the MOMP-QPLEX interaction and show by complementary in silico docking that QPLEX enters the porin channel through interactions at the extracellular face, translocates down the channel through a dipole transverse electric field towards the opposite end and is released into the periplasm at the intracellular face of MOMP. Our studies suggest a potential mechanism for the non-antibiotic anti-Campylobacter activity of these ferric chelates.
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影响因子：
16.6
作者：
Manzi, Lucio;Barrow, Andrew S.;Scott, Daniel;Layfield, Robert;Wright, Timothy G.;Moses, John E.;Oldham, Neil J.
通讯作者：
Oldham, Neil J.
影响因子：
4.4
作者：
Khattak F;Paschalis V;Green M;Houdijk JGM;Soultanas P;Mahdavi J
通讯作者：
Mahdavi J
影响因子：
5.7
作者：
Acosta-Gutierrez, Silvia;Scorciapino, Mariano Andrea;Ceccarelli, Matteo
通讯作者：
Ceccarelli, Matteo
影响因子：
5.8
作者：
Mahdavi, Jafar;Pirinccioglu, Necmettin;Ala'Aldeen, Dlawer A. A.
通讯作者：
Ala'Aldeen, Dlawer A. A.
DOI：
10.1039/c3mt20254b
发表时间：
2013-08
期刊：
Metallomics : integrated biometal science
影响因子：
--
作者：
Naikare H;Butcher J;Flint A;Xu J;Raymond KN;Stintzi A
通讯作者：
Stintzi A