Circulating blood monocytes traffic to and participate in the periprosthetic tissue inflammation.
Circulating blood monocytes traffic to and participate in the periprosthetic tissue inflammation.
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DOI：
10.1007/s00011-009-0051-5
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发表时间：
2009-12
影响因子：
6.7
通讯作者：
Yang, Shang-You
中科院分区：
文献类型：
--
作者：
Zhang, Kai;Jia, Tang-Hong;McQueen, David;Gong, Wei-Ming;Markel, David C.;Wooley, Paul H.;Yang, Shang-You
使用新型小鼠-人类嵌合体模型检查人类循环血单核细胞的运输及其对假体周围组织炎症的影响。通过定期腹腔注射抗去唾液酸 GM1 兔血清 (ASGM1)，将来自无菌假体松动患者的假体周围组织和骨碎片植入宿主巨噬细胞耗尽的免疫缺陷 SCID 小鼠的肌肉中。将患者自体外周血单核细胞 (PBMC) 用 PKH2 荧光染料标记并腹腔注射到植入的动物体内。 PBMC 输注后 14 天处死小鼠进行分子和组织学分析。 SCID 小鼠的患者假体周围组织具有良好的耐受性，并保持了高水平的活力。细胞运输研究揭示了异种移植物中荧光 PBMC 的积累，全身 PBMC 输注后异种移植物中的总细胞计数显着增加。 PBMC 输注还促进假体周围组织内 IL-1、IL-6、TNFα 和 RANK 的表达。全身PBMC迁移到植入的假体周围组织并导致局部炎症。这些数据证明循环血单核细胞可能在全关节置换术无菌松动的病理过程中发挥作用。
To examine the trafficking of human circulating blood monocytes and their influence on the inflammation of periprosthetic tissues using a novel mouse–human chimera model. Periprosthetic tissue and bone chips from patients with aseptic prosthetic loosening were implanted into the muscles of immune-deficient SCID mice depleted of host macrophages by periodic intraperitoneal injection of anti-asialo GM1 rabbit sera (ASGM1). Autologous patient peripheral blood monocytes (PBMCs) were labeled with PKH2 fluorescent dye and injected intraperitoneally into the implanted animals. Mice were sacrificed 14 days after PBMC transfusion for molecular and histological analyses. Patient periprosthetic tissues were well tolerated in SCID mice and preserved a high level of viability. Cell trafficking studies revealed the accumulation of fluorescent PBMC within the xenografts, with total cell counts in the xenografts significantly increased following the systemic PBMC infusion. PBMC infusion also promoted the expression of IL-1, IL-6, TNFα, and RANK within the periprosthetic tissue. Systemic PBMC migrated to the implanted periprosthetic tissues and contributed to the local inflammation. The data provide evidence that circulating blood monocytes may play a role in pathologic process during aseptic loosening of total joint replacement.
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DOI：
10.1097/00003086-199707000-00016
发表时间：
1997-07-01
影响因子：
4.2
作者：
Kadoya, Y;Revell, PA;Freeman, MAR
通讯作者：
Freeman, MAR
影响因子：
5.2
作者：
Askenasy, N;Farkas, DL
通讯作者：
Farkas, DL
DOI：
10.1084/jem.165.4.1212
发表时间：
1987-04-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Murphy WJ;Kumar V;Bennett M
通讯作者：
Bennett M
DOI：
10.2106/00004623-198365050-00001
发表时间：
1983-01-01
影响因子：
5.3
作者：
GOLDRING, SR;SCHILLER, AL;HARRIS, WH
通讯作者：
HARRIS, WH
影响因子：
--
作者：
Fang, Hsu-Wei;Ho, Yi-Ching;Huang, Chun-Hsiung
通讯作者：
Huang, Chun-Hsiung