Adenomatous polyposis coli (APC) protein moves along microtubules and concentrates at their growing ends in epithelial cells.
Adenomatous polyposis coli (APC) protein moves along microtubules and concentrates at their growing ends in epithelial cells.
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DOI：
10.1083/jcb.148.3.505
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发表时间：
2000-02-07
期刊：
影响因子：
--
通讯作者：
Tsukita S
中科院分区：
文献类型：
--
作者：
Mimori-Kiyosue Y;Shiina N;Tsukita S
结肠腺瘤性息肉病(APC)肿瘤抑制蛋白定位于迁移细胞边缘的微管末端附近。我们在非洲爪哇上皮细胞中表达了绿色荧光蛋白(GFP)与非洲爪哇APC全长和缺失突变体的融合蛋白，并观察了它们在活细胞中的动态行为。在细胞铺展和伤口愈合过程中，GFP标记的全长APC以颗粒状聚集在细胞延伸的顶端区域。在较高的放大倍数下，APC似乎沿MTS移动，并在生长的正端以颗粒状聚集。当MTS开始变短时，APC颗粒从MT末端脱落。免疫电子显微镜显示，MTS周围的模糊结构是这些GFP信号的超微结构对应。APC的COOH末端定位于生长中的MT末端，没有形成颗粒状聚集体，当MTS开始变短时，该区域突然消失。缺少COOH-末端区域的APC形成颗粒状聚集体，这些聚集体以依赖于ATP的方式沿MTS向其正端移动。这些发现表明，APC是一种独特的MT相关蛋白，它沿着选定的MT移动，并在其生长的正端通过其多个功能区进行浓缩。
Adenomatous polyposis coli (APC) tumor suppressor protein has been shown to be localized near the distal ends of microtubules (MTs) at the edges of migrating cells. We expressed green fluorescent protein (GFP)-fusion proteins with full-length and deletion mutants of Xenopus APC in Xenopus epithelial cells, and observed their dynamic behavior in live cells. During cell spreading and wound healing, GFP-tagged full-length APC was concentrated as granules at the tip regions of cellular extensions. At higher magnification, APC appeared to move along MTs and concentrate as granules at the growing plus ends. When MTs began to shorten, the APC granules dropped off from the MT ends. Immunoelectron microscopy revealed that fuzzy structures surrounding MTs were the ultrastructural counterparts for these GFP signals. The COOH-terminal region of APC was targeted to the growing MT ends without forming granular aggregates, and abruptly disappeared when MTs began to shorten. The APC lacking the COOH-terminal region formed granular aggregates that moved along MTs toward their plus ends in an ATP-dependent manner. These findings indicated that APC is a unique MT-associated protein that moves along selected MTs and concentrates at their growing plus ends through their multiple functional domains.
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DOI：
10.1073/pnas.90.23.11109
发表时间：
1993-12-01
影响因子：
11.1
作者：
JOSLYN, G;RICHARDSON, DS;ALBER, T
通讯作者：
ALBER, T
影响因子：
56.9
作者：
Matsumine, A;Ogai, A;Akiyama, T
通讯作者：
Akiyama, T
DOI：
10.1073/pnas.92.7.3046
发表时间：
1995-03-28
影响因子：
11.1
作者：
MUNEMITSU, S;ALBERT, I;POLAKIS, P
通讯作者：
POLAKIS, P
影响因子：
7.8
作者：
Diamantopoulos, GS;Perez, F;Rickard, JE
通讯作者：
Rickard, JE
影响因子：
8
作者：
Morrison, EE;Wardleworth, BN;Meredith, DM
通讯作者：
Meredith, DM