CMV on surfaces in homes with young children: results of PCR and viral culture testing.
CMV on surfaces in homes with young children: results of PCR and viral culture testing.
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DOI：
10.1186/s12879-018-3318-z
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发表时间：
2018-08-13
影响因子：
3.7
通讯作者：
Dollard SC
中科院分区：
文献类型：
--
作者：
Amin MM;Stowell JD;Hendley W;Garcia P;Schmid DS;Cannon MJ;Dollard SC
照顾幼儿是已知的巨细胞病毒（CMV）感染的危险因素，主要是通过接触他们的唾液和尿液。在之前的一项研究中，36名2岁至4岁的CMV血清阳性儿童根据唾液和尿液中CMV DNA的检测被分类为CMV脱落者（n = 23）或非脱落者（n = 13）。目前的研究评估了巨细胞病毒在儿童家中表面的存在。研究人员在100天内对36名参与研究的儿童进行了4次家访。用棉签擦拭口腔收集唾液，用插入尿布的滤纸收集尿液。此外，采集了5个表面标本：与儿童唾液接触的3个（勺子、儿童脸颊、毛巾）和与儿童尿液接触的2个（换尿布台、母亲的手）。通过PCR和病毒培养对样本进行检测，以定量CMV DNA和活病毒的存在。共检测了来自36个家庭的654个表面样本；136例CMV DNA阳性，其中122例（90%）来自CMV脱落儿童的家庭（p < 0.001）。唾液相关样本比尿液相关样本更常呈巨细胞病毒阳性，病毒载量更高。家庭中CMV病毒载量越高，PCR阳性（p = 0.01）和病毒培养阳性（p = 0.05）的家庭表面越多。家庭表面巨细胞病毒的主要来源是家庭中儿童的唾液。儿童的巨细胞病毒载量越高，表面上的活病毒越多，这可能有助于病毒传播。
Caring for young children is a known risk factor for cytomegalovirus (CMV) infection mainly through exposure to their saliva and urine. In a previous study, 36 CMV-seropositive children 2 mo. to 4 years old were categorized as CMV shedders (n = 23) or non-shedders (n = 13) based on detection of CMV DNA in their saliva and urine. The current study evaluated the presence of CMV on surfaces in homes of the children. Study staff made 4 visits to homes of the 36 enrolled children over 100 days. Saliva was collected by swabbing the mouth and urine was collected on filter paper inserted into diapers. In addition, five surface specimens were collected: three in contact with children’s saliva (spoon, child’s cheek, washcloth) and two in contact with children’s urine (diaper changing table, mother’s hand). Samples were tested by PCR and viral culture to quantify the presence of CMV DNA and viable virus. A total of 654 surface samples from 36 homes were tested; 136 were CMV DNA positive, 122 of which (90%) were in homes of the children shedding CMV (p < 0.001). Saliva–associated samples were more often CMV positive with higher viral loads than urine-associated samples. The higher the CMV viral load of the child in the home, the more home surfaces that were PCR positive (p = 0.01) and viral culture positive (p = 0.05). The main source for CMV on surfaces in homes was saliva from the child in the home. Higher CMV viral loads shed by children correlated with more viable virus on surfaces which could potentially contribute to viral transmission.
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影响因子：
5.1
作者：
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通讯作者：
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